Fasting venous blood samples were taken at study entry and collected in pre-cooled ethylenediaminetetra-acetic acid tubes after centrifugation at 1000 g for 15 min at 4℃ within 30 min of collection; all plasma aliquots were stored at −80℃. Total cholesterol (TC), LDL-C, HDL cholesterol (HDL-C), triglycerides (TG), ApoA1 and B, Lp(a), fasting glucose and high sensitivity C-reactive protein (hs-CRP) were assessed using commercial kits (Roche Diagnostics, Mannheim, Germany) and a Roche c701 analyser. Plasma PCSK9 levels were measured using an enzyme-linked immunosorbent assay (ELISA) with a commercial PCSK9 ELISA Kit (Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit; R&D Systems, Abingdon, UK).
Human proprotein convertase 9 pcsk9 quantikine elisa kit
Manufactured by R&D Systems
Sourced in United Kingdom, United States
The Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit is a quantitative sandwich enzyme immunoassay designed to measure human PCSK9 levels in cell culture supernatants, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Sunrise microplate reader, by Tecan (1 mentions)
Magellan software, by Tecan (1 mentions)
Infinity triglycerides reagent, by Thermo Fisher Scientific (1 mentions)
Infinity cholesterol reagent, by Thermo Fisher Scientific (1 mentions)
Mouse proprotein convertase 9 pcsk9 quantikine elisa kit, by R&D Systems (1 mentions)
4 protocols using human proprotein convertase 9 pcsk9 quantikine elisa kit
1
Fasting Lipid and PCSK9 Profiling
2
PCSK9 Quantification in Cell Lines
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PCSK9 was measured according to the manufacturer's instructions (Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit) (R&D Systems cat. #DPC900) in human LEC, HuH7 and HEK 293T supernatants that were concentrated using the Amicon® Ultra-2 centrifugal filter device (Milipore).
3
Quantification of Total Serum PCSK9 by ELISA
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Total PCSK9 was measured in serum samples using a sandwich enzyme-linked immunosorbent assay (ELISA) (Human Proprotein Convertase 9/PCSK9 Quantikine® ELISA Kit, R&D Systems, Minneapolis, MN, USA) on a Sunrise microplate reader and Magellan software (Tecan, Männedorf, Switzerland) as instructed by the manufacturer. Sensitivity and assay range provided by the manufacturer were 0.219 ng/mL and 0.6–40 ng/mL, respectively. Samples were diluted 1:20 with calibrator diluent. Samples exceeding the upper limit were diluted 1:40 and reanalyzed. The assay used measured total PCSK9 in serum, so it was not possible to distinguish between bound and unbound PCSK9.
4
Evaluating PCSK9 and ANGPTL3 Levels in Mice
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Initial blood samples were collected following a 4 h fast. Age-matched littermates/colonymates were randomly assigned to experimental groups and administered AAV particles (n = 5) via retro-orbital injection. Blood samples were collected following a 4 h fast at 1-week intervals via the tail tip. After 4 weeks, all mice were killed by carbon dioxide asphyxiation after a 4 h fast. Whole livers were collected for genomic DNA isolation and analysis and for hematoxylin/eosin staining, and terminal blood samples were collected.
Pre-treatment and post-treatment plasma human PCSK9, mouse Pcsk9 or mouse ANGPTL3 were measured using the human proprotein convertase 9/PCSK9 Quantikine ELISA kit, mouse proprotein convertase 9/PCSK9 Quantikine ELISA kit or human angiopoietin-like 3 Quantikine ELISA kit, respectively, according to the manufacturer’s instructions (R&D Systems). Total cholesterol or triglyceride levels were measured using the Infinity cholesterol reagent or Infinity triglycerides reagent, respectively, according to the manufacturer’s instructions (Thermo Fisher).
Pre-treatment and post-treatment plasma human PCSK9, mouse Pcsk9 or mouse ANGPTL3 were measured using the human proprotein convertase 9/PCSK9 Quantikine ELISA kit, mouse proprotein convertase 9/PCSK9 Quantikine ELISA kit or human angiopoietin-like 3 Quantikine ELISA kit, respectively, according to the manufacturer’s instructions (R&D Systems). Total cholesterol or triglyceride levels were measured using the Infinity cholesterol reagent or Infinity triglycerides reagent, respectively, according to the manufacturer’s instructions (Thermo Fisher).
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