Latrunculin b lat b

Manufactured by Cayman Chemical

Sourced in United States

Latrunculin B (Lat B) is a small molecule that binds to and sequesters actin monomers, inhibiting actin polymerization. It is a potent and reversible inhibitor of actin filament formation.

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Latrunculin B (15 citations) Latrunculin (2 citations)

2 protocols using latrunculin b lat b

Characterization of Efflux Transporter MRP4 in Cells

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MK571, H89, cAMP, RP-Adenosine 3′,5′-cyclic monophosphorothioate triethylammonium salt (Rp cAMPS), chlortetracycline (CTC), Hoechst 33258, Pissum sativum agglutinin-FITC (PSA-FITC), l-α-lysophosphatidylcholine (LPC) and bovine serum albumin (BSA) were acquired from Sigma-Aldrich (MO, USA). KT5720 was purchased from Tocris Bioscience (Bristol, UK). Latrunculin B (Lat B) was acquired from Cayman Chemical (MI, USA). Monoclonal antibody anti-MRP4 and anti-rabbit IgG coupled to Alexa Fluor 555 were obtained from Cell Signaling Technology (MA, USA) and Abcam (Cambridge, UK) respectively. Alexa Fluor 488-phalloidin, M199 medium, gentamicin and fungizone were purchased from Invitrogen (CA, USA). All other chemicals were of analytical grade and obtained from standard sources.

Chiarante N., Alonso C.A., Plaza J., Lottero-Leconte R., Arroyo-Salvo C., Yaneff A., Osycka-Salut C.E., Davio C., Miragaya M, & Perez-Martinez S. (2020). Cyclic AMP efflux through MRP4 regulates actin dynamics signalling pathway and sperm motility in bovines. Scientific Reports, 10, 15619.

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Cell Line Characterization and Culture

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IMR90, IMR90 E6E7 32 , and U-2OS cells were provided by Jan Karlseder (Salk Institute, La Jolla California), and HT1080 6TG cells by Eric Stanbridge (University of California, Irvine).
Cell line identity was verified by Cell Bank Australia using short tandem repeat profiling and all cell lines were identified as mycoplasma negative (MycoAlert, LT07-118, Lonza). All cells were grown at 37⁰ C, 10% CO2, and 3% O2 in DMEM (Life Technologies) supplemented with 1% non-essential amino acids (Life Technologies), 1% Glutamax (Life Technologies) and 1% penicillin-streptomycin (Life Technologies). IMR90, IMR90 E6E7 and IMR90 E6E7-FUCCI were supplemented with 10% fetal bovine serum (Life Technologies), and U-2OS and HT1080 6TG cultures with 10% bovine growth serum (HyClone). The following compounds were used in cell treatments: dimethyl sulfoxide (DMSO, Sigma-Aldrich), Aphidicolin (APH, Sigma-Aldrich), Hydroxyurea (HU, Sigma-Aldrich), Latrunculin B (LatB, Cayman Chemical: 10010631), INK128 (Cayman Chemical: 11811), VE-822 (Selleckchem: S7102) and Insulinlike growth factor-I (IGF1, Sigma-Aldrich: SRP4121,).

Lamm N., Read M.N., Nobis M., Van Ly D., Page S.G., Masamsetti V.P., Timpson P., Biro M, & Cesare A.J. (2020). Nuclear F-actin counteracts nuclear deformation and promotes fork repair during replication stress. Nature cell biology, 22(12).

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