Azadirachtin (purity, ≥97%) was purchased from Yunnan Zhongke Biological Industry Co., Ltd. (Kunming, China). SF9 cells were purchased from Qingqi Biotechnology Development Co., Ltd. High performance liquid chromatography (HPLC)-grade acetonitrile (J.T. Baker, Phillipsburg, NJ, USA) and ultra-pure water prepared from a Milli-Q purification system (Millipore, Burlington, MA, USA) were used for semipreparative HPLC analysis. Contact Angle and Surface Tension were measured using the JC2000C1 contact angle/surface tension measuring instrument (Shanghai Zhongchen Digital Technology Equipment Co., Ltd., Shanghai, China).
Electrospray ionization (ESI) was recorded on an Agilent 1290 UPLC/6540, and the 1D and 2D NMR spectra were measured on a Bruker 500 MHz spectrometer, with TMS as the internal standard. An RP-18 column (50 μm, YMC Co. Ltd., Kyoto, Japan) gel, MCI gel (75–150 μm, Sci-Bio Chem Co. Ltd., Chengdu, China), and Sephadex LH-20 (40–70 μm, Amersham Pharmacia Biotech AB, Uppsala, Sweden), were used for column chromatography. Semipreparative HPLC was performed on a YMC Luna C18 (5 μm; 10 × 250 mm) reversed-phase column.
Electrospray ionization (ESI) was recorded on an Agilent 1290 UPLC/6540, and the 1D and 2D NMR spectra were measured on a Bruker 500 MHz spectrometer, with TMS as the internal standard. An RP-18 column (50 μm, YMC Co. Ltd., Kyoto, Japan) gel, MCI gel (75–150 μm, Sci-Bio Chem Co. Ltd., Chengdu, China), and Sephadex LH-20 (40–70 μm, Amersham Pharmacia Biotech AB, Uppsala, Sweden), were used for column chromatography. Semipreparative HPLC was performed on a YMC Luna C18 (5 μm; 10 × 250 mm) reversed-phase column.