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Anti cd20 ecd clone b9e9

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The Anti-CD20 ECD (clone B9E9) is a laboratory reagent used for the detection and identification of CD20-positive cells. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the CD20 antigen expressed on the surface of B lymphocytes. This reagent can be used in flow cytometry applications to analyze and quantify CD20-positive cell populations.

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Lab products found in correlation

Anti cd3 alexa fluor 700 clone sp34.2, by BD (2 mentions) Anti cd45 v450 clone do58 1283, by BD (2 mentions) Hla dr pe clone l243 g46 6, by BD (2 mentions) Anti cd123 apc clone 7g3, by BD (2 mentions) Anti cd11c apc clone s hcl 3, by BD (2 mentions) Anti cd4 percp cy5.5 clone l200, by BD (2 mentions) Anti cd14 fitc clone m5e2, by BD (2 mentions) Anti cd8 apc h7 clone sk1, by BD (2 mentions) Rnaprotect, by Qiagen (2 mentions) Facsaria 2, by BD (2 mentions)

2 protocols using anti cd20 ecd clone b9e9

1

Isolation of PBMCs Subpopulations

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From anti-coagulated whole blood DCs, CD4+ T cells and B cells were sorted following staining with monoclonal antibodies to anti-CD45 V450 (clone DO58–1283; BD Pharmingen), anti-CD14 FITC (clone M5E2; BD Pharmingen), HLA-DR PE (clone L243 (G46–6); BD Pharmingen), anti-CD20 ECD (clone B9E9; Beckman Coulter), anti-CD4 PerCP Cy5.5 (clone L200; BD Pharmingen), anti-CD123 APC (clone 7G3; BD Pharmingen), anti-CD11c APC (clone S-HCL-3; BD Pharmingen), anti-CD3 Alexa Fluor 700 (clone SP34.2; BD Pharmingen), anti-CD8 APC.H7 (clone SK1; BD Pharmingen). After staining and washes, stained PBMCs were sorted using the BD FACSAria II. Doublets were excluded from analysis by gating singlets in forward scatter-area (FSC-A) versus forward scatter-height (FSC-H) and side scatter-area (SSC-A) versus side scatter-height (SSC-H) analysis. After gating on lymphocytes (CD45+ followed by FSC-A vs. SSC-A), total B lymphocytes were defined and sorted as CD3- CD8- CD20+ populations. Dendritic cells were gated and sorted as CD3- CD20- CD14- CD11c+/CD123+ cells. Sorted populations were collected into tubes containing RNA Protect (Qiagen) for RNA isolation.
Tomalka J.A., Pelletier A.N., Fourati S., Latif M.B., Sharma A., Furr K., Carlson K., Lifton M., Gonzalez A., Wilkinson P., Franchini G., Parks R., Letvin N., Yates N., Seaton K., Tomaras G., Tartaglia J., Robb M., Michael N., Koup R., Haynes B., Santra S, & Sekaly R.P. (2021). The transcription factor CREB1 is a mechanistic driver of immunogenicity and reduced HIV-1 acquisition following ALVAC vaccination. Nature immunology, 22(10), 1294-1305.
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2

Isolation of PBMCs Subpopulations

Check if the same lab product or an alternative is used in the 5 most similar protocols
From anti-coagulated whole blood DCs, CD4+ T cells and B cells were sorted following staining with monoclonal antibodies to anti-CD45 V450 (clone DO58–1283; BD Pharmingen), anti-CD14 FITC (clone M5E2; BD Pharmingen), HLA-DR PE (clone L243 (G46–6); BD Pharmingen), anti-CD20 ECD (clone B9E9; Beckman Coulter), anti-CD4 PerCP Cy5.5 (clone L200; BD Pharmingen), anti-CD123 APC (clone 7G3; BD Pharmingen), anti-CD11c APC (clone S-HCL-3; BD Pharmingen), anti-CD3 Alexa Fluor 700 (clone SP34.2; BD Pharmingen), anti-CD8 APC.H7 (clone SK1; BD Pharmingen). After staining and washes, stained PBMCs were sorted using the BD FACSAria II. Doublets were excluded from analysis by gating singlets in forward scatter-area (FSC-A) versus forward scatter-height (FSC-H) and side scatter-area (SSC-A) versus side scatter-height (SSC-H) analysis. After gating on lymphocytes (CD45+ followed by FSC-A vs. SSC-A), total B lymphocytes were defined and sorted as CD3- CD8- CD20+ populations. Dendritic cells were gated and sorted as CD3- CD20- CD14- CD11c+/CD123+ cells. Sorted populations were collected into tubes containing RNA Protect (Qiagen) for RNA isolation.
Tomalka J.A., Pelletier A.N., Fourati S., Latif M.B., Sharma A., Furr K., Carlson K., Lifton M., Gonzalez A., Wilkinson P., Franchini G., Parks R., Letvin N., Yates N., Seaton K., Tomaras G., Tartaglia J., Robb M., Michael N., Koup R., Haynes B., Santra S, & Sekaly R.P. (2021). The transcription factor CREB1 is a mechanistic driver of immunogenicity and reduced HIV-1 acquisition following ALVAC vaccination. Nature immunology, 22(10), 1294-1305.
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