Cd202b tie 2

Manufactured by BioLegend

CD202b (Tie-2) is a cell surface receptor that plays a role in the regulation of angiogenesis and vascular remodeling. It is expressed on endothelial cells and some tumor cells.

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Lab products found in correlation

Cd146, by BioLegend (2 mentions) Cd90 thy1, by BioLegend (2 mentions) Cd164, by BioLegend (2 mentions) Histopaque, by Merck Group (2 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (2 mentions) Facsaria 2, by BD (2 mentions)

2 protocols using cd202b tie 2

Isolation and Phenotyping of Human Skeletal Cells

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Human skeletal cells were separated from red blood cells (RBCs) and bone dust by density gradient separation using 1:1 Histopaque of density 1.119 g/mL (Sigma-Aldrich, St Louis, MO, USA). The buffy coat was collected, washed with staining media, and the resulting cell suspension was depleted of CD45 + cells by magnetic-activated cell sorting (MACS) (Miltenyi, Cat#130–045-801). Cells were blocked with mouse IgG and stained with fluorochrome-conjugated antibodies against CD45 (BioLegend, Cat#304029-BL), CD235a (BioLegend, Cat#306612-BL), CD31 (Thermo Fisher Scientific™, Cat#13–0319-82), CD202b (Tie-2) (BioLegend, Cat#334204), CD146 (BioLegend, Cat#342010), PDPN (Thermo Fisher Scientific™, Cat#17–9381-42), CD90 (THY1; BioLegend, Cat#328110), CD164 (BioLegend, Cat#324808), and CD73 (BioLegend, Cat#344016). Flow cytometry was performed on FACS Aria II (BD Biosciences). Gating schemes were established with fluorescence-minus-one (FMO: staining with all fluorophores except one) controls and negative propidium iodide (PI) (Sigma-Aldrich, Cat#P4170) staining (1 μg/ml) was used as a measure for cell viability.

Tevlin R., Griffin M., Chen K., Januszyk M., Guardino N., Spielman A., Walters S., Gold G.E., Chan C.K., Gurtner G.C., Wan D.C, & Longaker M.T. (2023). Denervation during mandibular distraction osteogenesis results in impaired bone formation. Scientific Reports, 13, 2097.

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Isolation and Characterization of Human Skeletal Cells

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Human skeletal cells were separated from red blood cells (RBCs) and bone dust by density gradient separation using 1:1 Histopaque of density 1.119 g/mL (Sigma-Aldrich, Cat#11191). The buffy coat was collected, washed with staining media, and the resulting cell suspension was depleted of CD45+ cells by magnetic-activated cell sorting (MACS) (Miltenyi, Cat#130–045-801). Cells were blocked with mouse IgG and stained with fluorochrome-conjugated antibodies against CD45 (BioLegend, Cat#304029-BL), CD235a (BioLegend, Cat#306612-BL), CD31 (Thermo Fisher Scientific™, Cat#13–0319-82), CD202b (Tie-2) (BioLegend, Cat#334204), CD146 (BioLegend, Cat#342010), PDPN (Thermo Fisher Scientific™, Cat#17–9381-42), CD90 (THY1; BioLegend, Cat#328110), CD164 (BioLegend, Cat#324808), and CD73 (BioLegend, Cat#344016). Flow cytometry was performed on FACS Aria II (BD Biosciences). Gating schemes were established with fluorescence-minus-one (FMO: staining with all fluorophores except one) controls and negative propidium iodide (PI) (Sigma-Aldrich, Cat#P4170) staining (1 μg/ml) was used as a measure for cell viability.

Chan C.K., Gulati G.S., Sinha R., Tompkins J.V., Lopez M., Carter A.C., Ransom R.C., Reinisch A., Wearda T., Murphy M., Brewer R.E., Koepke L.S., Marecic O., Manjunath A., Seo E.Y., Leavitt T., Lu W.J., Nguyen A., Conley S.D., Salhotra A., Ambrosi T.H., Borrelli M.R., Siebel T., Chan K., Schallmoser K., Seita J., Sahoo D., Goodnough H., Bishop J., Gardner M., Majeti R., Wan D.C., Goodman S., Weissman I.L., Chang H.Y, & Longaker M.T. (2018). Identification of the Human Skeletal Stem Cell. Cell, 175(1), 43-56.e21.

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