Sa β gal staining and reactive oxygen species assay kits

ASP was purchased from Ci Yun Biotechnology Co., Ltd. Shanxi (Xian, China). The component monosaccharide of ASP analyzed by high performance capillary electrophoresis were predominant arabinose, glucose, rhamnose, galactose, galacturonic acid as well as trace amount of mannose and glucuronic acid. D-galactose was purchased from Shanghai Puzhen Biological Science and Technology Co. Ltd. (Shanghai, China). MethoCultTM GF M3434 was from Stem Cell Technologies (Vancouver, BC, USA). The Anti-Sca-1 + Micro Bead Kit was purchased from Miltenyi Biotech Co. (Bergisch Gladbach, Germany). The SA-β-gal Staining and Reactive Oxygen Species Assay Kits were purchased from the Beyotime Institute of Biotechnology (Shanghai, China). The SOD and MDA kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The antibodies against p16, p53, p21, βactin were purchased from Santa Cruz (San Cruz, CA, USA), the antibodies against p-p38 and γH2AX were purchased from Cell Signaling Technology (Danvers, MA, USA), Cx43 antibody was purchased from Abcam (Cambridge, MA, USA), and SDF-1 antibody was purchased from Wuhan Boster Bio-engineering Co. Ltd. (Wuhan, China). Lucifer yellow was purchased from Sigma-Aldrich Inc. (St Louis, MO, USA). SCF, GM-CSF, IL-1β, IL-6, TNF-α, 8-OH-dG and RANTS ELISA Kits were purchased from Neobioscience Technology Company (Shenzhen, China).