Bone marrow-derived basophils were lysed with M-PER (Thermo Scientific) plus 1X halt protease inhibitor and EDTA (Thermo Scientific). Then, the samples were spun at 15,000xg for 15 min at 4 °C to remove cellular debris. Cell lysates were denatured by boiling for 10 min at 70 °C with 1X sample buffer (NuPage 4X LDS sample buffer, Life Technologies) and 1X reducing agent (NuPage 10X sample reducing agent, Life Technologies) and separated with SDS/PAGE (4–20% Bis-Tris-gels with MOPS running buffer, Genscript), electroblotted onto Invitrolon poly (vinylidene difluoride) membranes (Invitrogen), and then probed with a rat IgG2a anti-mouse Mcpt8 antibody (clone TUG8, catalog number 647401, BioLegend) along with a mouse IgG1 anti-GAPDH antibody (clone GA1R, Cell Sciences) as a loading control.
Mouse igg1 anti gapdh antibody clone ga1r
Manufactured by Cell Sciences
Mouse IgG1 anti-GAPDH antibody (clone GA1R) is a monoclonal antibody that binds to the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein.
Automatically generated - may contain errors
Lab products found in correlation
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (2 mentions)
Invitrolon polyvinylidene difluoride membranes, by Thermo Fisher Scientific (2 mentions)
Rat igg2a anti mouse mcpt8 antibody clone tug8, by BioLegend (2 mentions)
Nupage lds sample buffer 4x, by Thermo Fisher Scientific (2 mentions)
Nupage sample reducing agent 10x, by Thermo Fisher Scientific (2 mentions)
Bis tris gels with mops running buffer, by GenScript (2 mentions)
M per, by Thermo Fisher Scientific (2 mentions)
2 protocols using mouse igg1 anti gapdh antibody clone ga1r
1
Basophil Protein Expression Analysis
2
Basophil Protein Expression Analysis
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Bone marrow-derived basophils were lysed with M-PER (Thermo Scientific) plus 1X halt protease inhibitor and EDTA (Thermo Scientific). Then, the samples were spun at 15,000xg for 15 min at 4 °C to remove cellular debris. Cell lysates were denatured by boiling for 10 min at 70 °C with 1X sample buffer (NuPage 4X LDS sample buffer, Life Technologies) and 1X reducing agent (NuPage 10X sample reducing agent, Life Technologies) and separated with SDS/PAGE (4–20% Bis-Tris-gels with MOPS running buffer, Genscript), electroblotted onto Invitrolon poly (vinylidene difluoride) membranes (Invitrogen), and then probed with a rat IgG2a anti-mouse Mcpt8 antibody (clone TUG8, catalog number 647401, BioLegend) along with a mouse IgG1 anti-GAPDH antibody (clone GA1R, Cell Sciences) as a loading control.
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