C57bl 6j wild type mice wt

STING knockout mice (SKO) were generated in our laboratory (Ishikawa and Barber, Nature 2008) and have backcrossed with a C57BL/6J. Wild type C57BL/6J mice (WT) were purchased from Jackson Laboratory. cGAS Knockout mice (cGAS KO) were kindly provided by Dr. Herbert W. Virgin IV (Washington University, School of Medicine). TLR9 Knockout mice (TLR9KO) were purchased from Jackson Laboratory. Balb/C mice were purchased from Jackson Laboratory. To generate the conditioning Sting knockout mice, we developed animals with the STING gene floxed. In brief, the exons 1-5 were flanked with loxp sites in C57/BL6 derived embryonic stem (ES) cells in order to render STING susceptible to Cre-mediated recombination. The floxed STING mice were crossed to mice expressing Cre under a cell specific promoter (CD11C-Cre) to generate STING^loxp/CD11C-cre (CD11C-SKO) mice. Mice care and study were conducted under approval from the Institutional Animal Care and Use Committee of the University of Miami. Mouse genotypes from tail biopsies were determined by real-time PCR with specific probes designed for each gene by commercial vendor (Transnetyx).

Wild-type C57BL/6J mice (WT) were purchased from Jackson Laboratories (Bar Harbor, ME). ST2 KO mice were previously generated by Andrew McKenzie and backcrossed to C57BL/6J for 8 generations. IL-33 KO mice on the C57BL/6J background were provided by Dr. Dirk Smith (Amgen, Seattle, WA). IL-5–transgenic mice (strain NJ.1638, previously described (20 (link))) were provided to Dr. Sergejs Berdnikovs by Dr. James Lee (Mayo Clinic, Phoenix, AZ) and crossed with ST2 KO mice. Depending on the experimental requirements, both male and female mice (aged 6–36 weeks) were used. Animals were housed under specific pathogen–free conditions at Northwestern University. All experiments were approved by the Northwestern University Animal Care and Use Committee.

Mice in which a 90bp region of the Darc gene locus had been eliminated from exon II and bred on a C57Bl\6J background (Darc^ΔE2 mice) (15 (link)) were maintained as a breeding colony under pathogen-free conditions. Deletion of the 90kb region leads to loss of normal DARC mRNA expression and a substantially reduced affinity of erythrocytes to CXCL8 and CCL2 (15 (link)). Wild-type C57Bl\6J mice (WT) were purchased from Jackson Laboratories (Bar Harbor, ME, USA). Mice were studied at eight weeks of age and all experiments were approved by the Institutional Animal Care and Use Committee at the University of Vermont (#11-029).