Polymorphprep and lymphoprep reagents

Human neutrophils were isolated from healthy blood donors using density gradient centrifugation of polymorphprep and lymphoprep reagents (AXIS-SHIELD PoC AS, Oslo, Norway) according to the manufacture’s instruction. The regional ethics review board in Uppsala, Sweden has given their approval (Dnr 2015/437) to isolate blood from healthy individuals after informed consent. The collection of blood from healthy donors were performed according to the ethical guidelines of both the Declaration of Helsinki and the Swedish national board of health and welfare. The viability of neutrophils was examined by microscopy, as previously described^{22 (link)}.

Human neutrophils were isolated from healthy blood donors by density gradient centrifugation of Polymorphprep and Lymphoprep reagents (AXIS-SHIELD PoC AS, Oslo, Norway) or with the EasySep Human Neutrophil Enrichment Kit (STEMCELL Technologies, Cambridge, UK) according to the manufacturer’s instructions^{32 (link)}. The cells were infected in RPMI 1640 (Lonza, Basel, Switzerland) complemented with 10% heat inactivated fetal bovine serum (FBS) during the experiments. An ethical approval has been granted by the regional ethics review board in Uppsala, Sweden (Dnr 2015/437), to isolate blood from healthy individuals after informed consent. Blood from healthy donors were collected according to the ethical guidelines of both the Declaration of Helsinki and the Swedish national board of health and welfare. The viability of the neutrophils was > 90% as determined by the trypan blue exclusion test after isolation.