Hm 325

Manufactured by Leica

Sourced in Germany

The HM-325 is a high-quality microtome designed for sectioning a wide range of materials. It features precise thickness adjustment, a stable clamping system, and user-friendly operation.

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Lab products found in correlation

Axiovert 200m microscope, by Zeiss (1 mentions) Shandon citadel 2000, by Thermo Fisher Scientific (1 mentions) Axiocam hrc color camera, by Zeiss (1 mentions) Glass slide, by Matsunami (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Ultra turrax t25 basic, by IKA Group (1 mentions) Asp300, by Leica (1 mentions) Eg1160, by Leica (1 mentions) Tween 80, by Merck Group (1 mentions) Cremophor el, by Merck Group (1 mentions) Peg300, by Merck Group (1 mentions) Hi1210, by Leica (1 mentions) Absolute ethanol, by Chem-Supply (1 mentions) Peg 4000, by BD (1 mentions) Bx 40f 3, by Olympus (1 mentions) Propylparaben, by Merck Group (1 mentions) Methylparaben, by Merck Group (1 mentions)

Spelling variants of this product

HM 325 rotary microtome (2 citations)

3 protocols using hm 325

Decalcification and Paraffin Embedding of Bone Tissues

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Tibias, femurs, and humeri were obtained and fixed for 48 h in Carson’s Millonig formalin at room temperature, and mineralized bone decalcification was made through EDTA protocol (Carson et al., 1973 (link)). Samples were processed in a Shandon Citadel 2000 tissue processor (Thermo, Waltham, MA, USA) according to standard histological techniques for paraffin embedding. Paraffin sections with a thickness of 5 μm were obtained in a rotary microtome (Microm HM-325 or Leica RT2125). These sections were de-waxed in xylol and hydrated in decreasing ethanol concentrations until being washed in distilled water to prepare for staining with H&E and Sirius red. Slides with sections from tibias, femurs, and humeri were analyzed in an Axiovert 200M microscope and the images were acquired with an AxioCam HRc color camera (Carl Zeiss, Oberkochen, Germany).

Marins-Dos-Santos A., Ayres-Silva J.D., Antunes D., Moreira C.J., Pelajo-Machado M., Alfaro D., Zapata A.G., Bonomo A.C., Savino W., de Meis J, & Farias-de-Oliveira D.A. (2022). Oral Trypanosoma cruzi Acute Infection in Mice Targets Primary Lymphoid Organs and Triggers Extramedullary Hematopoiesis. Frontiers in Cellular and Infection Microbiology, 12, 800395.

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Tissue Preparation for Immunohistochemistry

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For immunohistochemistry and in situ hybridization, specimens were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (PB; pH 7.4) overnight at 4°C. Following fixation, preparations were dehydrated through graded ethanol concentrations, cleared in lemosol, embedded in paraffin wax, and sectioned on an HM 325 microtome (Leica, Wetzlar, Germany). Horizontal sections (7 μm in thickness) were mounted on glass slides (Matsunami Glass, Osaka, Japan).

Kobayashi A., Hamada M., Yoshida M.A., Kobayashi Y., Tsutsui N., Sekiguchi T., Matsukawa Y., Maejima S., Gingell J.J., Sekiguchi S., Hamamoto A., Hay D.L., Morris J.F., Sakamoto T, & Sakamoto H. (2022). Vasopressin-oxytocin–type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians. Science Advances, 8(9), eabk0331.

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Topical Anti-Inflammatory Formulation Development

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Materials used included stearyl alcohol (Scharlan, Spain), Vaseline® (Chem-supply, Australia), Span 40 and 80 (Aldrich, USA), Tween 80 (Aldrich, USA), absolute ethanol (Chem-supply, Australia), Polychol 15 (CRODA, Australia) Carbopol 934P (Neveon, USA), liquid paraffin (Ajax Finechem, New Zealand), polyethylene glycol (PEG) 4000 (BDH, England), PEG 300 (Sigma, Germany), Cremophor EL, Pluronic F-127, methyl paraben, propyl paraben, and 12-O-tetradecanoylphorbol-13-acetate (TPA) (Sigma, USA), acetone (Merck, Germany). For the HPLC mobile phase acetonitrile (HPLC grade) and formic acid (purity 98-100%) were purchased from Merck (Germany). Additional formulation excipients are listed separately in supplementary information. Equipment included a homogenizer (Ultra TURRAX® T25 basic, IKA Labortechnik), digital electronic micrometer (± 0.001 mm, Mitutoyo, Japan), Cyber Scan pH 1100 (Southern Cross, Science Pty Ltd, Australia), tissue processing machine (ASP300S, LEICA), tissue embedding machine (EG1160, LEICA), microtome (HM325, LEICA), tissue homogenizer (MICCRA D-1, ART-moderne Labortechnik, Germany), water bath (HI1210, LEICA), microscope (OLYMPUS BX 40F-3, Japan), centrifuge (Sigma 1-15, SIGMA Laborzentrifugen GmbH), mechanical tumbler (Erweka AR400, Erweka Apparatebau GMBH, Germany) and rotary evaporator (Buchi Rotavap RII).

Simpson B.S., Luo X., Wang J., Song Y., Claudie D., Garg S., Smith N., McKinnon R, & Semple S. (2015). Development and Evaluation of a Topical Anti-Inflammatory Preparation Containing Dodonaea polyandra Extract. Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 18(4).

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