Immunoblot analyses were performed using the following antibodies: FAK (Santa Cruz Biotechnology, Inc.), pFAKY397, Akt, pAktS473, HRP-linked anti-mouse IgG, HRP-linked anti-rabbit IgG (all Cell Signaling), IRDye® 680RD anti-mouse IgG, IRDye® 800CW anti-mouse IgG, IRDye® 680RD anti-rabbit IgG, and IRDye® anti-rabbit IgG (all LI-COR Biosciences). The mouse Abca1 antibody was a generous gift from Dr. David Castle (University of Virginia, Charlottesville, VA). Immunofluorescence staining was performed using rabbit anti-Salmonella (Thermo), donkey anti-rabbit AlexaFluor 488, and AlexaFluor 647 phalloidin (both Invitrogen).
Irdye anti rabbit igg
Manufactured by LI COR
The IRDye® anti-rabbit IgG is a secondary antibody conjugated with an infrared dye. It is designed to detect and quantify rabbit primary antibodies in Western blotting, immunohistochemistry, and other applications requiring infrared detection.
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Lab products found in correlation
Irdye 680rd anti rabbit igg, by LI COR (1 mentions)
Irdye 800cw anti mouse igg, by LI COR (1 mentions)
Irdye 680rd anti mouse igg, by LI COR (1 mentions)
Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions)
Hrp linked anti rabbit igg, by Cell Signaling Technology (1 mentions)
Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions)
P fak y397, by Cell Signaling Technology (1 mentions)
Pakt s473, by Cell Signaling Technology (1 mentions)
P stat3 tyr705, by Cell Signaling Technology (1 mentions)
Chameleon due pre stained protein ladder, by LI COR (1 mentions)
Irdye anti mouse igg, by LI COR (1 mentions)
2 protocols using irdye anti rabbit igg
1
Immunoblot and Immunofluorescence Protein Analysis
2
Western Blot Analysis of P-STAT3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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On ice, RIPA buffer was added to ASZs on a 10-cm plate, scrapped, and then incubated for 30 min. After lysis, samples were prepared through the addition SDS sample buffer (SDS loading buffer: 6× Laemmli SDS Sample buffer; Bioland Scientific; SAB03-01) and denaturated by heating at 95 °C for 15 min. Protein and protein ladder (Chameleon Due Pre-stained Protein Ladder; LI-COR; 928-60000) was then loaded into a precasted SDS gel (4–12% Bis-tris Precasted Gel NUPAGE; NP0321BOX) and ran in MOPs solution (NUPAGEl NP0001). Protein was transferred and then blocked with 5% milk at room temperature for 1 h. Blots were incubated with either P-Stat3 Tyr705 (Cell Signaling; 9145T; 1:1000) or Tubulin (DSHB; 4A1; 1:1000) overnight. Bands were visualized after staining with IRDye anti-Rabbit IgG (LI-COR; 926-68071; 1:10,000) and IRDye anti-Mouse IgG (LI-COR; 926-32210; 10000) on the Odyssey DLx.
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