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Peroxidase affinipure donkey anti rabbit igg h l antibody

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The Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody is a secondary antibody produced in donkeys and specifically recognizes the heavy and light chains of rabbit immunoglobulin G (IgG). The antibody is conjugated with the enzyme horseradish peroxidase (HRP), which can be used as a detection system in various immunoassay techniques.

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Lab products found in correlation

Bca assay, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Supersignal west pico plus chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) B4galt5 antibody, by Novus Biologicals (2 mentions) Gapdh antibody clone 14c10, by Cell Signaling Technology (2 mentions) Anti p24 antibody clone kc57, by Beckman Coulter (2 mentions) Fut6 antibody, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

AffiniPure Donkey Anti-Rabbit IgG (H + L) (15 citations) Peroxidase AffiniPure Donkey Anti-Rabbit IgG (12 citations) Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H + L) (7 citations) Donkey anti-Rabbit IgG (H+L); (4 citations) Rabbit anti-IgG (2 citations) Rabbit IgGs (2 citations)

2 protocols using peroxidase affinipure donkey anti rabbit igg h l antibody

1

Characterization of HIV Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
HIV-infected and uninfected H9 cells were pelleted and resuspended in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Thermo Scientific, cat# 87786). After centrifugation, the protein concentration in the cell lysates was determined using Bicinchoninic acid (BCA) assay (Thermo Scientific, cat# 23235). 1 μg of total protein lysates were resolved on a 4%–12% Bis-Tris gel at 200 V for 30 min. Resolved proteins were transferred to nitrocellulose membranes, blocked and probed with the following primary antibodies: human Fucosyltransferase 7/FUT7 antibody, (clone 795116, R&D Systems, cat# MAB64091), B4GALT5 antibody (Novus, cat# NBP2–14882), GAPDH antibody (clone 14C10, Cell Signaling Technology, cat# 2118), anti-p24 antibody (clone KC57, Beckman Coulter cat# 6604665), and FUT6 Antibody (Thermo Fisher, cat# PA5–96261). After two washes in 1X TBS supplemented with 0.05% Tween 20, membranes were incubated with HRP-conjugated secondary antibody: Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody (Jackson ImmunoResearch, cat# 711-035-152). Bound HRP-conjugated antibodies were revealed with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific, cat# 34580).
Colomb F., Giron L.B., Kuri-Cervantes L., Adeniji O.S., Ma T., Dweep H., Battivelli E., Verdin E., Palmer C.S., Tateno H., Kossenkov A.V., Roan N.R., Betts M.R, & Abdel-Mohsen M. (2020). Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy. Cell reports, 32(5), 107991.
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2

Characterization of HIV Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
HIV-infected and uninfected H9 cells were pelleted and resuspended in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Thermo Scientific, cat# 87786). After centrifugation, the protein concentration in the cell lysates was determined using Bicinchoninic acid (BCA) assay (Thermo Scientific, cat# 23235). 1 μg of total protein lysates were resolved on a 4%–12% Bis-Tris gel at 200 V for 30 min. Resolved proteins were transferred to nitrocellulose membranes, blocked and probed with the following primary antibodies: human Fucosyltransferase 7/FUT7 antibody, (clone 795116, R&D Systems, cat# MAB64091), B4GALT5 antibody (Novus, cat# NBP2–14882), GAPDH antibody (clone 14C10, Cell Signaling Technology, cat# 2118), anti-p24 antibody (clone KC57, Beckman Coulter cat# 6604665), and FUT6 Antibody (Thermo Fisher, cat# PA5–96261). After two washes in 1X TBS supplemented with 0.05% Tween 20, membranes were incubated with HRP-conjugated secondary antibody: Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) antibody (Jackson ImmunoResearch, cat# 711-035-152). Bound HRP-conjugated antibodies were revealed with SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific, cat# 34580).
Colomb F., Giron L.B., Kuri-Cervantes L., Adeniji O.S., Ma T., Dweep H., Battivelli E., Verdin E., Palmer C.S., Tateno H., Kossenkov A.V., Roan N.R., Betts M.R, & Abdel-Mohsen M. (2020). Sialyl-LewisX Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy. Cell reports, 32(5), 107991.
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