Mouse anti ezh2

Manufactured by BD

Mouse anti-EZH2 is a laboratory reagent used for the detection and quantification of the EZH2 protein in biological samples. EZH2 is a histone methyltransferase that plays a role in the regulation of gene expression.

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Lab products found in correlation

Vinculin, by Merck Group (1 mentions) Tubb3, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated anti mouse and anti rabbit igg, by Cytiva (1 mentions) H3k27me3, by Merck Group (1 mentions) Actin, by Merck Group (1 mentions) Histone h3, by Abcam (1 mentions) Anti rabbit igg, by Cell Signaling Technology (1 mentions) Anti mouse igg, by Cell Signaling Technology (1 mentions) Rabbit anti c myc, by Abcam (1 mentions) Rabbit anti cleaved caspase 3, by Abcam (1 mentions) Rabbit anti ha tag, by Abcam (1 mentions) Rabbit anti parp, by Abcam (1 mentions) Rabbit anti h3k27me3, by Abcam (1 mentions) Rabbit anti ezh2, by Cell Signaling Technology (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions) Rabbit anti h3, by Abcam (1 mentions)

2 protocols using mouse anti ezh2

Western Blot Analysis of Molecular Markers

Cited 1 time

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Whole-cell extracts were obtained for SDS-PAGE electrophoresis as previously described (25 (link)). Primary antibodies used were rabbit anti-SMARCA4 (Abcam, ab110641), H3K27Me3 (Millipore, 07–449), Myc (Abcam, ab32072), mouse anti-EZH2 (BD Bioscience, 612667), vinculin (Sigma, v9264), Actin (Sigma, A5441), Histone H3 (Abcam, ab1791), MAP2 (Millipore, MAB3418), TUBB3 (Santa Cruz, sc-51670), BAD (Santa Cruz, sc-8044), p16 (Ventana, 725–4713) and CDKN1A/p21 (BD Pharmacogen, 556430) and horseradish peroxidase-conjugated anti-mouse and anti-rabbit IgG (Amersham). Chemiluminescence was used for detection (Perkin–Elmer Life Sciences). Actin or vinculin were used as protein loading controls.

Wang Y., Chen S.Y., Colborne S., Lambert G., Shin C.Y., Santos N.D., Orlando K.A., Lang J.D., Hendricks W.P., Bally M.B., Karnezis A.N., Hass R., Underhill T.M., Morin G.B., Trent J.M., Weissman B.E, & Huntsman D.G. (2018). Histone deacetylase inhibitors synergizes with catalytic inhibitors of EZH2 to exhibit anti-tumor activity in small cell carcinoma of the ovary, hypercalcemic type. Molecular cancer therapeutics, 17(12), 2767-2779.

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Chromatin Remodeling Protein Antibody Panel

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Antibodies used in the work include mouse anti-EZH2 (BD, Cat # 612666), Sheep anti-EED (R&D, Cat # AF5827), rabbit anti-H3 (Abcam, Cat # ab1791), rabbit anti-EZH2 (Cell Signaling Technology (CST), Cat # 5246), rabbit anti-cMyc (CST, Cat # 5605), rabbit anti-H3K27me3 (CST, Cat # 9733), rabbit anti-HA tag (CST, Cat # 3724), rabbit anti-GAPDH (CST, Cat # 5174), rabbit anti-SUZ12 (Abcam, Cat # ab12073), rabbit anti-PHF19 (CST, Cat # 77271), rabbit anti-IKZF1 (CST, Cat # 9034), rabbit anti-IKZF3 (CST, Cat # 15103), rabbit anti-CRBN (CST, Cat # 71810), rabbit anti-PARP (CST, Cat # 9532), rabbit anti-Cleaved Caspase-3 (CST, Cat # 9661), rabbit anti-Cleaved Caspase-7 (CST, Cat # 8438). Normal Rabbit IgG (CST, Cat # 2729). HRP-linked secondary antibodies, either anti-mouse IgG (Cat # 7076) or anti-rabbit IgG (Cat # 7074), were obtained from Cell Signaling Technology. Sheep IgG HRP-conjugated Antibody (R&D, Cat # HAF016) were used as secondary antibody.

Purcell M., Mabrouk Y.M, & Bogorad L. (1995). Red/far-red and blue light-responsive regions of maize rbcS-m3 are active in bundle sheath and mesophyll cells, respectively.. Proceedings of the National Academy of Sciences of the United States of America, 92(25), 11504-11508.

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