The following instructions were given to the individuals. Two hours before salivary collections, the participants were requested to avoid eating food and drinking beverages. Saliva was collected during morning hours. The sample collection was as per a study by Chiu et al.,[17 (link)] where they emphasized to “spit clear saliva into an Eppendorf tube to minimize the amount of mucous.” Following transportation to the laboratory, samples were centrifuged at 3000 rpm for 5 min and stored at −'20°C. After thawing, 20 μl of salivary samples were transferred to a sample cup, and cortisol was estimated using a cortisol RP Elecsys kit (Roche Diagnostics, USA) in the cobas e 411 analyzer. Various studies[18 (link)19 ] have reported that salivary cortisol levels were found to be highly correlated with serum cortisol.
Cortisol rp elecsys kit
Manufactured by Roche
Sourced in United States
The Cortisol RP Elecsys kit is a laboratory testing product from Roche that is used to measure the concentration of cortisol, a hormone, in a patient's blood sample. The kit provides a quantitative determination of cortisol levels.
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Lab products found in correlation
3 protocols using cortisol rp elecsys kit
1
Salivary Cortisol Collection and Analysis
2
Salivary Cortisol Awakening Response Measurement
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Salivary cortisol was collected at three time-points to determine the cortisol awakening response (CAR): immediately after awakening and 30 and 45min thereafter (Powell and Schlotz, 2012 (link)). All samples were then stored at 4°C until sent to the laboratory. Once in the laboratory, saliva samples were centrifuged for 5-min at 2000 rpm to extract saliva with low viscosity, and subsequently transferred to 1.5 mL tubes and stored in a freezer at −80°C until analysis. After thawing, 20 μl of salivary samples were transferred to a sample cup and cortisol was estimated using a cortisol RP Elecsys Kit (Roche Diagnostics, United States) in a cobas e 411 analyzer. Summary indexes of CAR included the area under the curve with respect to ground (AUCg) and the area under the curve with respect to increase (AUCi) (Pruessner et al., 2003 (link)).
3
Salivary Cortisol Awakening Response Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Salivary cortisol was collected at three time points to determine the cortisol awakening response (CAR): immediately after awakening and 30 and 45 min thereafter (Powell and Schlotz, 2012 (link)). All samples were stored at 4°C until sent to the laboratory, where they were then centrifuged for 5 min at 2,000 rpm to extract saliva with low viscosity and subsequently transferred to 1.5-ml tubes and stored in a freezer at –80°C until analysis. After thawing, 20 μl of salivary samples was transferred to a sample cup and cortisol was estimated using a cortisol RP Elecsys kit (Roche Diagnostics, United States) in a cobas e 411 analyzer. Summary indexes of CAR included the area under the curve with respect to ground (AUCG) and the area under the curve with respect to increase (AUCI) calculated according to the work of Pruessner et al. (2003) (link).
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