Sw948

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SW948 is a centrifuge designed for high-speed separation of laboratory samples. It features a compact benchtop design and can achieve centrifugal forces up to 30,000 x g. The SW948 is suitable for a variety of applications that require efficient sample separation and purification.

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Lab products found in correlation

5 protocols using sw948

Comprehensive Colorectal Cancer Cell Line Profiling

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Human colon epithelial cell line NCM460 and human CRC cell lines Lovo, SW48, T84, SW948, LS174T, HT29, HCT116 and SW480 were purchased from Procell Life Sciences Co. Ltd., (Wuhan, Hubei, China). Cell lines NCM460 was cultured in RPMI-1640 (Gibco) medium, T84 in DMEM/F12 (Gibco) medium, Lovo in F12K (Gibco) medium, LS174T in MEM, HT29 and HCT116 in (Gibco) McCoy's 5A, SW48, SW948 and SW480 in L15 (Gibco) medium.
All media for the human cell lines were supplemented with 10% FBS and 1% penicillin/streptomycin antibiotics. SW48, SW948 and SW480 cell lines were cultured in 100% air at 37 °C. Other cell lines were cultured under 95% air-5% CO₂ at 37 °C. All cell lines were validated by STR DNA finger-printing. Experiments were carried out within 6 months after acquisition of the cell lines. In addition, mycoplasma contamination was ruled out using a PCR-based method.
HT29 and LS174T cells were transfected with miR-20a mimic and negative control (NC) or miR-20a inhibitor and inhibitor NC (inNC) respectively using Lipofectamine 2000 (Invitrogen, USA). After 48 h transfection, these cells were harvested for further experiments. These inhibitors and activator, including C75 (inhibitor of FASN), PKI-402 (inhibitor of PI3K/mTOR), MSAB (inhibitor of Wnt/β-catenin), Mycro 3 (inhibitor of Myc) and ZLN024 (activator of AMPK), were purchased from GLPBIO.

Song K., Liu C., Zhang J., Yao Y., Xiao H., Yuan R., Li K., Yang J., Zhao W, & Zhang Y. (2022). Integrated multi-omics analysis reveals miR-20a as a regulator for metabolic colorectal cancer. Heliyon, 8(3), e09068.

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Characterization of Colorectal and Cancer Cell Lines

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Colorectal cancer (CRC) cell lines DLD1, HCT116, RKO, SW480, LoVo, SW948, H460, T47D, HEK 293 T were purchased from the ATCC. Colorectal cancer (CRC) cell lines DLD1, HCT116, RKO, SW480, LoVo, SW948and genetically engineered isogenic cell lines DLD1 PIK3CA E545K cells and DLD1 PIK3CA WT cells were grown in McCoy’s 5 A medium (Gibco) supplemented with 10% of fetal bovine serum (Gibco). Lung cancer cell line H460 and breast cancer cell line T47D were cultured in RPMI 1640 medium (Sigma) containing 10% of FBS. Breast cancer cell line MDA-MB361 was maintained in Leibovitz’s L-15 medium (Gibco) with 20% of FBS. Human embryonic kidney HEK 293 T cells were cultured in DMEM medium (Sigma) containing 10% FBS. Penicillin/Streptomycin (1%) was added to tissue culture media for all cultures. Cells were incubated at 37 °C in a humidified atmosphere with 5% CO₂. All cell lines were tested routinely to avoid Mycoplasma contamination (Yeasen, cat # 40601ES20). The cell lines were authenticated by the Genetica DNA Laboratories using STR profiling. Transfection was conducted using Lipofectamine 3000 reagent (Life Technologies) according to the manufacturer’s instructions.

Hao Y., He B., Wu L., Li Y., Wang C., Wang T., Sun L., Zhang Y., Zhan Y., Zhao Y., Markowitz S., Veigl M., Conlon R.A, & Wang Z. (2022). Nuclear translocation of p85β promotes tumorigenesis of PIK3CA helical domain mutant cancer. Nature Communications, 13, 1974.

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Validation of Colorectal Cancer Cell Lines

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Human colon epithelial cell line NCM460 and human CRC cell lines Lovo, SW48, T84, SW948, LS174T, HT29, HCT116 and SW480 were purchased from Procell Life Sciences Co. Ltd., (Wuhan, Hubei, China).
Cell lines NCM460 was cultured in RPMI-1640 (Gibco) medium, T84 in DMEM/F12 (Gibco) medium, Lovo in F12K (Gibco) medium, LS174T in MEM, HT29 and HCT116 in (Gibco) McCoy's 5A, SW48, SW948 and SW480 in L15 (Gibco) medium.
All media for the human cell lines were supplemented with 10% FBS and 1% penicillin/streptomycin antibiotics. SW48, SW948 and SW480 cell lines were cultured in 100% air at 37 °C. Other cell lines were cultured under 95% air-5% CO 2 at 37 °C. All cell lines were validated by STR DNA nger-printing.
Experiments were carried out within 6 months after acquisition of the cell lines. In addition, mycoplasma contamination was ruled out using a PCR-based method.
HT29 and LS174T cells were transfected with miR-20a mimic and negative control (NC) or miR-20a inhibitor and inhibitor NC (inNC) respectively using Lipofectamine 2000 (Invitrogen, USA). After 48 h transfection, these cells were harvested for further experiments.

Song K., Liu C., Zhang J., Yao Y., Xiao H., Yuan R., Li K., Yang J., Zhao W., & Zhang Y. (2021). Integrated Multi-Omics Analysis of Colorectal Cancer Reveals Mir-20a as a Regulator of Fatty Acid Metabolism in Consensus Molecular Subtype 3.

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Culturing Colon Cancer Cell Lines

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The colon cancer cell lines (HCT116, COLO320, T24, HT29, SW480, SW948 and SW1417) used in our study were purchased from American Type Culture Collection (ATCC). The colon cancer cell line COLO678 was obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). Human HEK293T was purchased from ATCC. The culture medium for SW480 and HEK293T was RPMI-1640 medium (Invitrogen, Carlsbad, California, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, Utah, USA), 100 U/mL Penicillium and 100 μg/mL Streptomycin. The culture medium for HCT116, COLO320, T24, HT29, SW480, SW948 and SW1417 were DMEM: F12 (1:1) medium (Thermo Fisher Scientific, Waltham, Massachusetts, USA) supplemented with 10% fetal bovine serum (Hyclone, Logan, Utah, USA), 100 U/mL Penicillium and 100 μg/mL Streptomycin. Cells were maintained at 37 °C in a humidified atmosphere with 5% CO₂. Replaced the culture medium 2–3 times every week.

Fu D., Ren Y., Wang C., Yu L, & Yu R. (2021). LINC01287 facilitates proliferation, migration, invasion and EMT of colon cancer cells via miR-4500/MAP3K13 pathway. BMC Cancer, 21, 782.

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Culturing Colorectal Cancer Cell Lines

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Colon cancer cell lines COLO320, SW480, SW1417, SW948, T84, HT29 and human HEK293T cell line were obtained from American Type Culture Collection (ATCC). Colon cancer cell line COLO678 and CL11 were purchased from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). SW480, COLO678 and HEK293T cells were cultured with RPMI-1640 medium (Invitrogen, USA). COLO320, SW1417, SW948, T84, HT29 and CL11 cells were cultured with DMEM: F12 (1:1) medium (Thermo Fisher Scientific, USA). All cells were supplemented with 10% fetal bovine serum (Hyclone, USA) and 1% PenStrep (100 U/mL Penicillium and 100 μg/mL Streptomycin) in a humidified incubator containing 5% CO₂ at 37°C.

Sun T., Liu Z., Zhang R., Ma S., Lin T., Li Y., Yang S., Zhang W, & Wang Y. (2020). Long Non-Coding RNA LEF1-AS1 Promotes Migration, Invasion and Metastasis of Colon Cancer Cells Through miR-30-5p/SOX9 Axis. OncoTargets and therapy, 13, 2957-2972.

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