Anti phospho hsp27 ser82

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-phospho-HSP27 (Ser82) is a primary antibody that specifically recognizes the phosphorylated form of HSP27 (Heat Shock Protein 27) at serine 82. This antibody is designed for use in Western blotting and other immunodetection applications.

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Lab products found in correlation

Anti hsp27, by Cell Signaling Technology (2 mentions) Anti cpla2, by Cell Signaling Technology (1 mentions) Purified rat anti mouse ifn γ, by BD (1 mentions) Ovalbumin a5503, by Merck Group (1 mentions) Biotin rat anti mouse il 5, by BD (1 mentions) Purified rat anti mouse igg1 a85 3, by BD (1 mentions) Purified rat anti mouse il 4, by BD (1 mentions) Purified rat anti mouse il 5, by BD (1 mentions) Biotin rat anti mouse ige r35 118, by BD (1 mentions) Biotin rat anti mouse il 4, by BD (1 mentions) Biotin rat anti mouse ifn γ, by BD (1 mentions) Purified rat anti mouse ige r35 72, by BD (1 mentions) Anti apoa1, by Cell Signaling Technology (1 mentions) Anti rabbit cy3, by Jackson ImmunoResearch (1 mentions) Eclipse ti e inverted microscope system, by Nikon (1 mentions) Anti mapkapk 2, by Cell Signaling Technology (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (1 mentions) Anti nanog, by Proteintech (1 mentions) Anti sox2, by Proteintech (1 mentions)

Spelling variants of this product

HSP27 (39 citations) Phospho-HSP27 (16 citations) Anti-HSP27 (15 citations) Phospho-HSP27 (Ser82) (3 citations) Anti-phospho HSP27 (3 citations)

4 protocols using anti phospho hsp27 ser82

Antibody Reagents for Immunological Assays

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All commercial antibodies and reagents were purchased from the following resources: anti-phospho-cPLA2 (Ser505) substrate (#2831, 1: 1000 dilution), anti-cPLA2 (#2832, 1: 1000 dilution), anti-phospho-HSP27 (Ser82) (#2401, 1: 1000 dilution), anti-HSP27 (#2402, 1: 1000 dilution), and anti-ApoA1 (#3350, 1: 1000 dilution) antibodies were from Cell Signaling Technology. Ovalbumin (A5503) was purchased from Sigma-Aldrich (St. Louis, USA). Purified rat anti-mouse IgE (R35-72), purified rat anti-mouse IgG1 (A85-3), purified rat anti-mouse IgG2a (R11-89), purified rat anti-mouse IL-4, purified rat anti-mouse IL-5, purified rat anti-mouse IFN-γ, biotin rat anti-mouse IgE (R35-118), biotin rat anti-mouse IgG1 (A85-1), biotin rat anti-mouse IgG2a (19-5), biotin rat anti-mouse IL-4, biotin rat anti-mouse IL-5, and biotin rat anti-mouse IFN-γ were purchased from BD Biosciences (San Diego, USA).

Lee Y., Hwang Y.H., Kim K.J., Park A.K., Paik M.J., Kim S.H., Lee S.U., Yee S.T, & Son Y.J. (2017). Proteomic and transcriptomic analysis of lung tissue in OVA-challenged mice. Archives of Pharmacal Research, 41(1), 87-100.

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Dual-Immunostaining of Cardiac Tissue

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Frozen histological LV sections (n = 3/group) were fixed, blocked and dual-stained with anti-PKD (Sigma-Aldrich; dilution 1:200) or anti-phospho-HSP27 (Ser 82) (Cell Signaling Technology; 1:50) and anti-α-actinin (sarcomere; Sigma-Aldrich; dilution 1:400) antibody, and were incubated with the appropriate secondary antibodies: (FITC) anti-mouse (Rockland Immunochemicals Inc, Limerick, PA, USA; dilution 1:300) and Cy3 anti-rabbit (Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; dilution 1:100). Immunostained samples were analyzed by confocal laser scanning microscopy (Nikon Eclipse Ti-E Inverted Microscope System; Nikon Instruments, Nikon Corp, Shinagawa, Tokyo, Japan). Immunofluorescence imaging was processed equally among groups, for 2D intensity histogram analysis the Coloc2 plugin in FIJI was used.

Herwig M., Kolijn D., Lódi M., Hölper S., Kovács Á., Papp Z., Jaquet K., Haldenwang P., Dos Remedios C., Reusch P.H., Mügge A., Krüger M., Fielitz J., Linke W.A, & Hamdani N. (2020). Modulation of Titin-Based Stiffness in Hypertrophic Cardiomyopathy via Protein Kinase D. Frontiers in Physiology, 11, 240.

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Protein Expression Analysis of Stem Cell Markers

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Total protein was collected using a protein extraction kit (CWBIO, China). A BCA protein assay kit (Pierce Biotechnology, USA) was used to determine the concentrations of proteins. Western blotting was performed as described previously.^{40 (link)} The antibodies used in this study included anti-WIP1 (Abcam, USA, ab31270), anti-p38 (Abcam, ab32142), anti-phospho-p38 MAPK (Cell Signaling Technology, USA, #4511), anti-MAPKAPK-2 (Cell Signaling Technology, #3042), anti-phospho-MAPKAPK-2 (Thr222) (Cell Signaling Technology, #3316), anti-phospho-MAPKAPK-2 (Thr334) (Cell Signaling Technology, #3041), anti-HSP27 (Cell Signaling Technology, #2402), anti-phospho-HSP27 (Ser82) (Cell Signaling Technology, #9709), anti-SOX2 (Proteintech, USA, 11064-1), anti-OCT4 (Proteintech, 11263-1), anti-NANOG (Proteintech, 14295-1), anti-ALDH1A1 (Santa Cruz, USA, sc-374149), and anti-β-actin (Santa Cruz, sc-47778). Antibodies against p38 isoforms (p38α, β, γ, and δ) have been described previously.^{41 (link)}

Deng K., Liu L., Tan X., Zhang Z., Li J., Ou Y., Wang X., Yang S., Xiang R, & Sun P. (2020). WIP1 promotes cancer stem cell properties by inhibiting p38 MAPK in NSCLC. Signal Transduction and Targeted Therapy, 5, 36.

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Molecular Mechanisms of FoxO3 Regulation

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CTX, cycloheximide and PF-3644022 were purchased from Sigma-Aldrich. The plasmids pcDNA3-Myc6-ERK3-Flag, pcDNA3-Myc6-ERK3, pcDNA3-MK5-HA, pcDNA3-MK5-Flag, pcDNA3-MK5-HA L337A have been previously described (4, 28, 45) . pcDNA3-FoxO3-Myc was a kind gift of A. Brunet (Stanford University). pcDNA3-FoxO3-Myc S214A was generated by PCR mutagenesis. To construct the bacterial expression plasmid pGEX-mFoxO3, mouse FoxO3 was amplified by PCR using pcDNA3-FoxO3-Myc as template and subcloned into the EcoRI site of pGEX-KG. All mutations and PCR products were verified by DNA sequencing.
Commercial antibodies were obtained from the following suppliers and used at the indicated concentrations: anti-ERK3 (1/1000; cat. no. ab53277) and anti-myogenin (1/1000; cat. no. 232558) from Abcam; anti-MK5 (1/500; cat. no. sc-46667), anti-Myc (1/1000; cat. no. sc-40) and anti-Hsc70 (1/2000; cat. no. sc-7298) from Santa-Cruz Biotechnology; anti-MHC (1/1000; cat. no. MF20) and anti-Pax7 (1/100) from DSHB; anti-FoxO3 (1/1000; cat. no. 2497) and anti-phospho-Hsp27(Ser82) (1/1000; cat. no.2406) from Cell Signaling Technology; anti-HA (1/1000; cat. no. 901501) from Biolegend; anti-Flag (1/1000; cat. no. F3165), anti-laminin (1/500; cat. no. L9393) and anti-actin (1/1000; cat. no. A4700) from Sigma-Aldrich.

Soulez M., Tanguay P., Dô F., Crist C., Dort J., Kotlyarov A., Gaestel M., Dumont N.A., & Meloche S. (2021). ERK3-MK5 signaling regulates myogenic differentiation and muscle regeneration by promoting FoxO3 degradation.

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