Log In Sign up
The largest database of trusted experimental protocols

Neutrophil elastase

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Neutrophil Elastase is an enzyme found in the azurophilic granules of neutrophils. It plays a key role in the degradation of extracellular matrix proteins, contributing to the immune response and tissue remodeling.

Automatically generated - may contain errors

Lab products found in correlation

Histone h3, by Cell Signaling Technology (1 mentions) P jnk jnk, by Cell Signaling Technology (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) P iκb, by Cell Signaling Technology (1 mentions) Lipopolysaccharide lps from e coli 055 b5, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Human il 8, by R&D Systems (1 mentions) Inf γ, by R&D Systems (1 mentions) Human il 6 elisa kit, by Elabscience (1 mentions) Il 1β, by R&D Systems (1 mentions) Il 10, by R&D Systems (1 mentions) Vs200 slide scanner, by Olympus (1 mentions) Eclipse e800 microscope, by Nikon (1 mentions)

2 protocols using neutrophil elastase

1

Dehydrozingerone Attenuates Inflammatory Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dehydrozingerone (> 99% purity) bought from TCI chemicals and structure of the compound was shown in Fig. S1A, Lipopolysaccharide (LPS from E. Coli 055: B5) purchased from Sigma Aldrich (USA). Dexamethasone was purchased from Sigma-Aldrich, USA. C-DNA synthesis kit, SYBR green mix purchased from Takara bioscience INDIA. Antibodies GAPDH, JNK P-JNK, c-JUN, p-NF-κB, NF-κB, p38, pP38, Histone-H3, IκB, p-IκB, MPO, Neutrophil Elastase were purchased from Cell signalling technology (USA), ELISA kits (IL-6, CCL2, IL-10, INF-γ, Human-IL-8 and IL-1β were bought from R&D systems. Human IL-6 ELISA kit was procured from Elabsciences (Houston, USA). Secondary Antibodies were procured from Jackson Immuno research private limited (USA). TUNEL kit was procured from Merk Millipore.
Tirunavalli S.K., Gourishetti K., Kotipalli R.S., Kuncha M., Kathirvel M., Kaur R., Jerald M.K., Sistla R, & Andugulapati S.B. (2021). Dehydrozingerone ameliorates Lipopolysaccharide induced acute respiratory distress syndrome by inhibiting cytokine storm, oxidative stress via modulating the MAPK/NF-κB pathway. Phytomedicine, 92, 153729.
+ Open protocol
+ Expand
2

Quantifying Immune Cell Populations

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as previously described21 (link). Briefly, frozen section slides were thawed, fixed with 10% paraformaldehyde, blocked, and incubated with antibodies to CD68, CD14, and neutrophil elastase (Cell Signaling) for macrophage, monocyte, and neutrophil staining respectively. Macrophage images were analyzed at 20X magnification with a Nikon E800 Eclipse microscope (Nikon, Tokyo, Japan). Monocyte and neutrophil images were analyzed at 20X magnification with an Olympus VS200 Slide Scanner (Olympus, Tokyo, Japan). Macrophages were counted for each specimen at each high-powered field and averaged for each specimen. Monocytes and neutrophils were counted for each specimen using QuPath software.
Sabe S.A., Scrimgeour L.A., Karbasiafshar C., Sabra M., Xu C.M., Aboulgheit A., Abid M.R, & Sellke F.W. (2022). Extracellular Vesicles Modulate Inflammatory Signaling in Chronically Ischemic Myocardium of Swine with Metabolic Syndrome. The Journal of thoracic and cardiovascular surgery, 165(5), e225-e236.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!