TMAs and slides were stained for subtype‐defined markers of SCLC including ASCL1 (clone 24B72D11.1, dilution 1:100; BD Biosciences), NEUROD1 (clone EPR17084, dilution 1:50; Abcam), POU2F3 (clone 6D1, dilution 1:100; Santa Cruz), YAP1 (clone 63.7, dilution 1:2000; Santa Cruz) and Vimentin (clone D21H3, dilution 1:100; Cell Signaling Technologies) using Anti‐mouse/rabbit IHC Detection Kit (PK10006; Proteintech) according the manufacturer's protocol. Two independent pathologists reviewed the stained slides in a blinded fashion. The expression of each marker from tumor cells was assessed by histoscore (H‐score, range 0–300), which was calculated by multiplying the proportion of positive tumor cells (0%–100%) by the intensity of positive staining (no staining = 0, weak staining = 1, moderate staining = 2, and strong staining = 3).37 , 38 A dominant marker is defined as the marker with the highest H‐score. In SCLC with combined SCLC and NSCLC components, IHC scores reflect expression exclusively in the SCLC component.
Yap1 clone 63
Manufactured by Santa Cruz Biotechnology
Sourced in United States
YAP1 (clone 63.7) is an antibody product from Santa Cruz Biotechnology. It recognizes the YAP1 protein. The core function of YAP1 is to act as a transcriptional regulator that plays a role in the Hippo signaling pathway.
Automatically generated - may contain errors
2 protocols using yap1 clone 63
1
Immunohistochemical Profiling of SCLC Subtypes
2
Immunohistochemical Analysis of SCLC Subtypes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
TMAs and slides were stained for subtype-defined markers of SCLC including ASCL1 (clone 24B72D11.1, dilution 1:100, BD Biosciences, New York, USA), NEUROD1 (clone EPR17084, dilution 1:50, Abcam, Cambridge, UK), POU2F3 (clone 6D1, dilution 1:100, Santa Cruz, Texas, USA) and YAP1 (clone 63.7, dilution 1:2000, Santa Cruz) using Anti-mouse/rabbit Immunohistochemistry (IHC) Detection Kit (Cat No. PK10006, Proteintech, Chicago, USA) according to the manufacture protocol. Two independent pathologists reviewed the stained slides in a blinded fashion. The expression of each marker was assessed by histoscore (H-score, range, 0–300), which was calculated by multiplying the proportion of positive cells (0–100%) by the intensity of positive staining (no staining =0, weak staining =1, moderate staining =2, and strong staining =3). And the molecular subtype for a tumor was assigned based on the highest H-score among subtyping markers (15 (link)).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!