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Complete megm

Manufactured by Lonza
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Sourced in Switzerland

Complete MEGM is a cell culture medium designed for the growth and maintenance of human mammary epithelial cells. It provides the necessary nutrients and growth factors required for optimal cell growth and proliferation.

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Lab products found in correlation

Ultra low attachment 96 well plate, by Corning (2 mentions) Accutase, by Thermo Fisher Scientific (1 mentions) Ivision software, by Abcam (1 mentions)

Spelling variants of this product

MEGM complete media (3 citations) MEGM complete medium (2 citations)

3 protocols using complete megm

1

Sphere Formation Assay for CSCs

Cited 1 time
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10 (OCTT2, CAL33) or 20 (SCC9) cells/well were cultured in serum-free complete MEGM (Lonza, Basel, Switzerland) on ultralow attachment 96-well plates (Corning, Corning, NY) as detailed by us previously [28 (link)]. Spheres were counted 14 days after plating.
Facompre N.D., Harmeyer K.M., Sahu V., Gimotty P.A., Rustgi A.K., Nakagawa H, & Basu D. (2017). Targeting JARID1B’s demethylase activity blocks a subset of its functions in oral cancer. Oncotarget, 9(10), 8985-8998.
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2

Culturing Breast Cancer Cell Lines

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Breast cancer MDA-MB-231 cells were maintained in Dulbecco’s Modified Eagle’s Medium (ATCC) supplemented with 10% fetal bovine serum and penicillin/streptomycin. MCF10A cells were maintained in complete MEGM (Lonza, Houston, TX, USA). All cell cultures were incubated at 37 °C with 5% CO2 in a humidified incubator.
Khazal K.F, & Hill D.L. (2015). Withania somnifera extract reduces the invasiveness of MDA-MB-231 breast cancer and inhibits cytokines associated with metastasis. Journal of cancer metastasis and treatment, 1(2), 94-100.
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3

Mammosphere Formation and Propagation

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10 cells/well were cultured in ultralow attachment 96-well plates (Corning, Corning, NY) in serum-free complete MEGM (Lonza, Basel, Switzerland) for 14 days. Spheres were counted and imaged using a Leica DM IRB inverted microscope and iVision software (Biovision, Chester Springs, PA). Spheres were propagated by Accutase® (Life Technologies) dissociation for 20 minutes and trypan blue dead cell exclusion before re-plating.
Facompre N.D., Harmeyer K.M., Sole X., Kabraji S., Belden Z., Sahu V., Whelan K., Tanaka K., Weinstein G.S., Montone K.T., Roesch A., Gimotty P.A., Herlyn M., Rustgi A.K., Nakagawa H., Ramaswamy S, & Basu D. (2016). JARID1B enables transit between distinct states of the stem-like cell population in oral cancers. Cancer research, 76(18), 5538-5549.
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