Nahpo4 12h2o

Manufactured by Nacalai Tesque

Sourced in Japan

NaHPO4·12H2O is a chemical compound that is primarily used as a laboratory reagent. It is the sodium salt of phosphoric acid, containing 12 water molecules per formula unit. This compound is commonly utilized in various scientific and analytical applications that require a stable source of sodium and phosphate ions.

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Lab products found in correlation

Potassium chloride (kcl), by Nacalai Tesque (2 mentions) Kh2po4, by Nacalai Tesque (2 mentions) Penicillin streptomycin, by Nacalai Tesque (2 mentions) Sodium chloride (nacl), by Fujifilm (2 mentions) T 2252, by Merck Group (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Hypoxanthine, by Merck Group (1 mentions) Holo tf, by Merck Group (1 mentions)

2 protocols using nahpo4 12h2o

Formulation of PBS Supplemented Buffer

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PBS (–) was dissolved 0.8% NaCl (Fujifilm Wako Chemicals, Osaka, Japan), 0.02% KCl (Nacalai Tesque, Kyoto, Japan), 0.28% NaHPO₄·12H₂O
(Nacalai Tesque) and 0.02% KH₂PO₄ (Nacalai Tesque) in ultrapure water. PBS (+) was dissolved 0.1% CaCl₂ (Hayashi Pure Chemical
Ind., Osaka, Japan), 0.1% MgCl₂·6H₂O (Katayama Chemical Industries, Osaka, Japan) and 1% glucose (Nacalai Tesque) in ultrapure water. PBS
was dissolved 9% PBS (–), 10% PBS (+), 1% PVP (Sigma-Aldrich, St. Lous, MO, USA) and 0.2% penicillin streptomycin (Nacalai Tesque) in ultrapure water.

TONAI S., KAWABATA A., NAKANISHI T., LEE J.Y., OKAMOTO A., SHIMADA M, & YAMASHITA Y. (2020). Iron deficiency induces female infertile in order to failure of follicular development in mice. The Journal of Reproduction and Development, 66(5), 475-483.

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Porcine Oocyte Maturation Media Preparation

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PBS (−) was dissolved in 0.8% (w/v) NaCl (Fujifilm Wako Chemicals), 0.02% (w/v) KCl (Nacalai Tesque), 0.28% (w/v) NaHPO₄·12H₂O (Nacalai Tesque), 0.02% (w/v) KH₂PO₄ (Nacalai Tesque), and ultrapure water. PBS (+) was dissolved in 0.1% (w/v) CaCl₂ (Hayashi Pure Chemical Ind., Ltd), 0.1% (w/v) MgCl₂·6H₂O (Katayama Chemical Industries Co, Ltd), 1% (w/v) glucose (Nacalai Tesque), and ultrapure water. PBS was dissolved in 9% (v/v) PBS (−), 10% (v/v) PBS (+), 1% (v/v) PVP (Sigma‐Aldrich), 0.2% (v/v) penicillin streptomycin (Nacalai Tesque), and ultrapure water. Modified NCSU37 (mNCSU37) medium was used as basic medium for pre‐IVM and IVM of porcine COCs.⁵, ²³, ²⁴ Highly purified FSH was a kind gift from the National Hormone and Pituitary Program (National Institute of Diabetes, Digestive and Kidney Diseases; NIDDK). Hypoxanthine (Sigma‐Aldrich) was dissolved in the mNCSU37. Holo‐TF (monoferric or diferric TF; T8158) and Apo‐TF (non‐iron bond TF; T2252) were purchased for Sigma‐Aldrich and were dissolved in the mNCSU37. Fetal calf serum (FCS) was purchased from Invitrogen.

Tonai S., Nakanishi T., Yamaoka M., Okamoto A., Shimada M, & Yamashita Y. (2023). Pre‐culture with transferrin‐Fe3+ before in vitro maturation improves the developmental competence of porcine oocytes matured in vitro. Reproductive Medicine and Biology, 22(1), e12529.

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