ChIP-seq libraries were prepared with the KAPA Library Preparation Kit (KK8234, Kapa Biosystems) and NETflex DNA barcodes (514104, Bioo Scientific). A total of 10 mg of DNA was used as starting material for input and immunoprecipitation samples. Libraries were amplified with a thermocycler for 13 cycles. Postamplification libraries were size-selected at 250 to 45 bp in length using the Agencourt AMPure XP beads (A63881, Beckman Coulter). Libraries were validated using the Agilent High Sensitivity DNA Analysis Kit (5067-4626). Libraries were sequenced at 30 million reads on the Illumina NextSeq 500 Sequencing System.
Netflex dna barcodes
Manufactured by PSC Biotech
The NETflex DNA barcodes are a laboratory tool designed for labeling and identification purposes. They consist of a unique sequence of DNA that can be used to track and differentiate samples. The core function of the NETflex DNA barcodes is to provide a reliable method for sample identification and traceability.
Automatically generated - may contain errors
2 protocols using netflex dna barcodes
1
ChIP-seq Library Preparation and Sequencing
2
ChIP-Seq Library Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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ChIP-Seq libraries were prepared with the KAPA Library preparation kit (KK8234, Kapa Biosystems) and NETflex DNA barcodes (514104, Bioo Scientific). A total of 10 µg DNA was used as starting material for input and immunoprecipitation samples. Libraries were amplified with a thermocycler for 13 cycles. Post-amplification libraries were size-selected at 250-45 bp in length using the Agencourt AMPure XP beads (A63881, Beckman Coulter). Libraries were validated using the Agilent High Sensitivity DNA Analysis Kit (5067-4626). ChIP-Seq libraries were single-read sequenced on the Illumina NextSeq 500 Sequencing System.
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