Pixma mp250

A collagen matrix was set up using 1.8 mg/ml sterile collagen and a neutralization solution (35 (link)). The neutralization solution was made by mixing Waymouth medium (Sigma, W1625) and 2 parts 0.34 M NaOH (Sigma, 221465). One part neutralization mixture was then added to 4 parts of collagen, mixed with 1 × 10⁵ cells to a final volume of 500 μl, and added to each well in a 24-well plate. After a 45-min incubation at 37°C, 1 ml 2% FBS was added to each well, and the plate was incubated for an additional 72 h at 37°C. The medium was then removed, fresh medium and treatment were added, and the collagen matrix was released using a sterile spatula. The plate was scanned using a Canon PIXMA MP250 immediately after release and also at 1, 3, 5, and 24 h. The size of the collagen matrix was measured using ImageJ, and the percentage of contraction was calculated. To decrease any shock to the myofibroblasts, all bacterial strains were grown in DMEM with 2% FBS.