The total RNA was isolated from SCCHN tissues and cell lines using the TRIzol reagents (Invitrogen, Carlsbad, CA, USA), and cDNA was reverse transcribed using the All-in-One™ mRNA cDNA synthesis Kit (GeneCopoeia, Rockville, MD, USA) following the manufacturers’ protocols. Relative MKRN3 expression values were calculated using the 2−△△CT method and normalized using the GAPDH expression levels [14 (link), 15 (link)]. The primers used are listed in Additional file 1 : Table S1.
All in one mrna cdna synthesis kit
Manufactured by GeneCopoeia
Sourced in United States
The All-in-One™ mRNA cDNA Synthesis Kit is a laboratory product designed for the reverse transcription of mRNA to cDNA. It provides a simple and efficient method for the conversion of mRNA to complementary DNA (cDNA) in a single-tube reaction.
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Lab products found in correlation
2 protocols using all in one mrna cdna synthesis kit
1
Quantitative RNA Expression Analysis
2
MKRN3 Expression Analysis in SCCHN
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The total RNA was isolated from SCCHN tissues and cell lines using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA), and cDNA was reverse transcribed using the All-in-One™ mRNA cDNA synthesis Kit (GeneCopoeia, Rockville, MD, USA) following the manufacturers' protocols. Relative MKRN3 expression values were calculated using the 2 -△△CT method and normalized with GAPDH as a reference [11, 12] . The primers are listed in Supplementary Table 1.
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