Anti cd31 phycoerythrin pe

Expression of the cell surface markers CD73, CD90 and CD105 and absence of hematopoietic markers on MSCs was determined using flow cytometry, according to the statement by the International Society for Cellular Therapy [30 (link)]. Cells were detached using Trypsin/EDTA and washed in PBS/0.05% bovine serum albumin. Antibodies used were (FITC) conjugated anti-CD86-fluorescein isothiocyanate (FITC) (cat. no. 555657), anti-HLA-DR-FITC (cat. no. 347400), anti-CD31-phycoerythrin (PE) (cat no 555446), anti-CD34-PE (cat no 348057), anti-CD73-PE (cat no 550257), anti-CD90-PE (cat no 555596), anti-CD3-peridinin chlorophyll protein(PerCP)-Cy5.5 (cat no 332771), anti-CD45-PerCPCy5.5 (cat no 332784), all from BD (San Diego, CA, USA) and anti-CD105-PE (cat no SN6), from Ancell (Bayport, MN, USA). Flow cytometry was performed on a FACScalibur, and analyzed using Cellquest software (both Becton–Dickinson). Mean fluorescence intensity (MFI) ratio was calculated by determining the MFI of the specific staining relative to the MFI of the appropriate isotype control staining.