Rabbit anti pt18 s19 mlc2

Manufactured by Cell Signaling Technology

Rabbit anti-pT18/S19 MLC2 is a primary antibody that recognizes phosphorylated myosin light chain 2 (MLC2) at threonine 18 and serine 19 residues. It can be used to detect the phosphorylation status of MLC2 in various experimental applications.

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2 protocols using rabbit anti pt18 s19 mlc2

Protein Signaling Pathway Antibodies

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Rabbit anti-Akt1 (2H10), rabbit anti-pT308Akt (244F9), rabbit anti-pS241PDK1, rabbit anti-PDK1, rabbit anti-pS19MLC2, rabbit anti-pT18/S19 MLC2, and rabbit anti-MLC2 were purchased from Cell Signaling Technology. Rabbit anti-MRCKα (H-90), goat anti-actin (C-11), mouse anti-GST (B-14), goat anti-ROCK1 (K-18), mouse anti–c-Myc (SC-40), and mouse anti-PDK1 (E-3) were from Santa Cruz Biotechnology, Inc. Mouse anti-FLAG and mouse anti-CDC42BPB clone 2F4 (anti-MRCKβ) were from Sigma-Aldrich. Rabbit anti-pT696MyPT1 was from EMD Millipore. Rabbit anti-MyPT1 was from EMD Millipore. Rabbit anti-GFP was from Life Technologies. Human EGF was purchased from R&D Systems. Human HGF was from PeproTech. LY294002 was from Cell Signaling Technology. Y-27632 was from EMD Millipore. Chelerythrine chloride was from Sigma-Aldrich. GSK2334470 was provided by the MRC Protein Phosphorylation Unit. Alexa Fluor 647, 555, and 488 Phalloidin were obtained from Invitrogen.

Gagliardi P.A., di Blasio L., Puliafito A., Seano G., Sessa R., Chianale F., Leung T., Bussolino F, & Primo L. (2014). PDK1-mediated activation of MRCKα regulates directional cell migration and lamellipodia retraction. The Journal of Cell Biology, 206(3), 415-434.

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Molecular Markers of Cell Signaling

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Rabbit anti-pS19MLC2, rabbit anti-pT18/S19 MLC2, and rabbit cleaved caspase 3 (Asp175) antibodies were purchased from Cell Signaling Technology. Mouse monoclonal anti-MRCKα antibody and mouse anti-GST (B-14) were from Santa Cruz Biotechnology. Mouse anti-CDC42BPB clone 2F4 (anti-MRCKβ) was from Sigma-Aldrich. Rabbit anti-GFP was from Thermo Fisher Scientific. Phalloidin-Atto 488, Phalloidin-Atto 594, blebbistatin, doxorubicin hydrochloride, ML-7 (MLCK inhibitor), Y-27632, tBid inhibitor BI-6C9, cytochalasin D, and latrunculin A were purchased from Sigma-Aldrich. zVAD-FMK was purchased from Merck-Millipore.

Gagliardi P.A., Somale D., Puliafito A., Chiaverina G., di Blasio L., Oneto M., Bianchini P., Bussolino F, & Primo L. (2018). MRCKα is activated by caspase cleavage to assemble an apical actin ring for epithelial cell extrusion. The Journal of Cell Biology, 217(1), 231-249.

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