Mirnase

Manufactured by Qiagen

MiRNase is a laboratory equipment product designed for the purification and isolation of microRNA (miRNA) from various biological samples. It provides a reliable and efficient method for extracting miRNA from cells, tissues, or body fluids. The core function of MiRNase is to enable the separation and concentration of miRNA molecules for subsequent analysis and research applications.

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Lab products found in correlation

Taqman microrna probe, by Thermo Fisher Scientific (2 mentions) Taqman microrna expression assay, by Thermo Fisher Scientific (2 mentions) Microrna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using mirnase

Quantitative miRNA Expression Analysis

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Encapsulated miRNAs were extracted using miRNase (Quiagen) according to the manufacturer’s instruction. From making template to quantification of miRNAs were performed as previously described (15 (link)). Briefly, the templates were made from 10 ng of total RNA using microRNA reverse transcription kit (Life Technologies) with specific primers, which are provided with TaqMan microRNA probes (Life technologies). Real-time PCR quantification for miRNA expression was performed using a TaqMan microRNA expression assay from Life Technologies. Cq value was converted to relative number using power formulation.

Eguchi A., Lazaro R.G., Wang J., Kim J., Povero D., Willliams B., Ho S.B., Stärkel P., Schnabl B., Ohno-Machado L., Tsukamoto H, & Feldstein A.E. (2016). Extracellular vesicles released by hepatocytes from gastric infusion model of ALD contain a miRNA barcode that can be detected in blood. Hepatology (Baltimore, Md.), 65(2), 475-490.

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Quantifying Cellular microRNA Levels

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Encapsulated miRNAs were extracted using miRNase (Quiagen) according to the manufacturer’s instruction. The quantities of miRNAs were assessed as previously described [24 (link)]. Briefly, the templates were made from 10 ng of total RNA using microRNA reverse transcription kit (Life Technologies) with specific primers, which are provided with TaqMan microRNA probes (Life technologies). Real-time PCR quantification for miRNA expression was performed using a TaqMan microRNA expression assay from Life Technologies. Cq value was converted to relative number using ddCt (delta delta Ct) model.

Eguchi A., Lazic M., Armando A.M., Phillips S.A., Katebian R., Maraka S., Quehenberger O., Sears D.D, & Feldstein A.E. (2016). Circulating adipocyte-derived extracellular vesicles are novel markers of metabolic stress. Journal of molecular medicine (Berlin, Germany), 94(11), 1241-1253.

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