AQX-1125 was purchased from MedChemExpress (MCE). The primary antibodies of GAPDH, PI3K, p-PI3K, Akt, p-AKT, P65, p-P65, IκBα, p- IκBα, SHIP1, Runx2, Alp, NFATc1, and c-Fos were acquired from ABclonal (Wuhan, China). Phalloidin and 4, 6-diamidino-2-phenylindole (DAPI) were purchased from Solarbio (Beijing, China). RANKL (the receptor activator of the nuclear factor kappa-B ligand) and M-CSF (macrophage colony-stimulating factor) were obtained from R&D Systems (Minnesota, USA). Cell culturing plates were purchased from NEST (Jiangsu, China). Minimum Essential Medium Alpha (α-MEM), Dulbecco’s Modified Eagle Medium: F-12 (DMEM/F-12), fetal bovine serum (FBS), penicillin, streptomycin, and trypsin were purchased from Gibco (Grand Island, NY, United States). The TRAP staining kit was obtained from Solarbio (Beijing, China).
P iκbα
Manufactured by ABclonal
Sourced in China
P-IκBα is a lab equipment product used for detecting and quantifying the phosphorylation of IκBα, a key regulator of the NF-κB signaling pathway. It is designed for use in various research applications, such as Western blotting and ELISA, to study the activation and regulation of the NF-κB pathway.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Trap staining kit, by Solarbio (1 mentions)
C fos, by ABclonal (1 mentions)
P pi3k, by ABclonal (1 mentions)
P akt, by ABclonal (1 mentions)
Runx2, by ABclonal (1 mentions)
Nfatc1, by ABclonal (1 mentions)
M csf, by R&D Systems (1 mentions)
Rankl, by R&D Systems (1 mentions)
Gapdh, by ABclonal (1 mentions)
Ipvh00005, by Merck Group (1 mentions)
Ap0707, by ABclonal (1 mentions)
Goat anti rabbit igg h l, by ABclonal (1 mentions)
A19056, by ABclonal (1 mentions)
Staining kit, by Beyotime (1 mentions)
Rat il 1β, by Merck Group (1 mentions)
Tgf β1, by ABclonal (1 mentions)
Adamts 4, by ABclonal (1 mentions)
3 protocols using p iκbα
1
Osteoclastogenesis Regulation by AQX-1125
2
Protein Extraction and Western Blot Analysis
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Cellular proteins were isolated using radio-immuno precipitation assay (RIPA) buffer (P0013B, Beyotime, Shanghai, China), followed by separation via 8% sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and their transfer onto polyvinylidene fluoride (PVDF) membranes (IPVH00005, Millipore, Billrica, MA, USA). Afterward, the membranes underwent blocking with 5% Bovine Serum Albumin (BSA) for 1 hour, followed by PBS washing. Subsequently, the membranes underwent an overnight incubation at 4 °C with the respective primary antibodies: p-IκBα (AP0707, 1:1000, ABclonal, Wuhan, China), NF-κB (A14754, 1:1000, ABclonal, Wuhan, China), Notch1 (A7636, 1:1000, ABclonal, Wuhan, China), Hes-1 (A0925, 1:1000, ABclonal, Wuhan, China), GAPDH (A19056, 1:5000, ABclonal, Wuhan, China). After washing with PBS, the membranes underwent incubation with the corresponding secondary anti-body, goat anti-rabbit IgG (H+L) (AS014, 1:2000, ABclonal, Wuhan, China), for 2 hours at room temperature. After three washes with tris-buffered saline Tween-20 (TBST) buffer (60145ES76, Yi Sheng Biotechnology, Shanghai, China) for 5 minutes each, signal detection was performed utilizing an enhanced chemiluminescence detection kit (P0018S, Beyotime, Shanghai, China). Image J software (V1.8.0.112, NIH, Madison, WI, USA) was used for image analysis.
3
Inflammatory Cytokine Regulation in Rat Cells
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MT, Rat IL-1β, 4′,6-diamidino-2-phenylindole (DAPI), collagenase type II, and EX527 were purchased from Sigma Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) (BI, Kibbutz Bet Hamek, Israel), Dulbecco’s Modified Eagle’s Medium-(DMEM-) F12 Medium, and Phosphate Buffered Saline purchased from Corning (New York, NY, USA). 1% penicillin/streptomycin, cell counting kit-8 (CCK-8), and staining kit were purchased from Beyotime (Haimen, China). Trypsin (0.25%) was purchased from Gibco (Grand Island, NY, USA). Lysate and RAPI were purchased from Biyuntian (Shanghai, China). nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (p65), Phosphorylated nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor (p-p65),alpha (IκBα), p-IκBα, TGF-β1, Smad2, iNOS, COX-2, matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), collagen type II alpha 1 chain (COL2A1), SIRT1, and ADAMTS-4 were purchased from ABclonal Technology (Wuhan, China). Horseradish peroxidase-conjugated goat anti-mouse/rabbit immunoglobulin G (IgG) secondary antibody was purchased from ABclonal Technology (UK). GAPDH was purchased from CST (Danvers, MA, USA).
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