Eclipse e800 system

Cupriavidus necator strain KK10 was isolated from a diesel fuel-degrading bacterial consortium that originated from cattle pasture soil from the Gulf region of Texas by streaking on solid medium with a heavy fraction of diesel fuel as the carbon and energy source (21 (link), 22 (link), 40 (link), 50 (link), 51 (link)). Strain KK10 was routinely cultured on 20 mM glycerol in Stainier’s basal medium (SBM) at 30°C with rotary shaking at 150 rpm in the dark. The growth capability of strain KK10 on the aromatic hydrocarbon substrates as sole carbon and energy sources was tested by incubating bacterial cells under the same conditions with 50 mg L⁻¹ of selected substrates in 20 mL of SBM in 100-mL-volume conical flasks that were stoppered with silicone plugs. Cell growth was evaluated by measuring the optical density of the cultures at 600 nm, and light microscopy was utilized to observe motility and cell multiplication using an Eclipse E800 system (Nikon, Tokyo, Japan).

Cupriavidus necator strain KK10 was isolated from a diesel fuel-degrading bacterial consortium that originated from cattle pasture soil from the Gulf region of Texas by streaking on solid medium with a heavy fraction of diesel fuel as the carbon and energy source (21 (link), 22 (link), 40 (link), 50 (link), 51 (link)). Strain KK10 was routinely cultured on 20 mM glycerol in Stainier’s basal medium (SBM) at 30°C with rotary shaking at 150 rpm in the dark. The growth capability of strain KK10 on the aromatic hydrocarbon substrates as sole carbon and energy sources was tested by incubating bacterial cells under the same conditions with 50 mg L⁻¹ of selected substrates in 20 mL of SBM in 100-mL-volume conical flasks that were stoppered with silicone plugs. Cell growth was evaluated by measuring the optical density of the cultures at 600 nm, and light microscopy was utilized to observe motility and cell multiplication using an Eclipse E800 system (Nikon, Tokyo, Japan).