About 600 µL of transfected protoplasts were aliquoted in 24-multiwell plates. At each time point 99 µL of the treated protoplasts were mixed with 1 µL of fluorescein diacetate (1 µg mL−1; Sigma-Aldrich), incubated 10 min in the dark, and imaged with a Leica THUNDER Imager Model Organism stereo microscope. Viability was calculated as the percentage of fluorescein diacetate-stained intact protoplasts. The remaining 500 µL of protoplasts were then used for luciferase activity quantification.
Thunder imager model organism stereo microscope
Manufactured by Leica
The THUNDER Imager Model Organism stereo microscope is a high-performance imaging system designed for the observation and analysis of small model organisms. The microscope provides a wide field of view and high-resolution imaging capabilities, allowing users to capture detailed images and videos of their samples.
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Lab products found in correlation
2 protocols using thunder imager model organism stereo microscope
1
Protoplast Viability and Luciferase Assay
2
Histochemical Analysis of Plant Roots
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Histochemical analysis was performed using 1-cm sections of roots that were cut from the root body and immobilised in 7% agarose blocks. Transverse 200 μM sections were then obtained using a TPI Vibratome Series 1000 Classic. Plant material was dehydrated by washing in growing concentration of ethanol (30/50/75%) for 5 min each and stained with 0.02% toluidine blue. Root slices were then rinsed with distilled water to remove the excess staining solution. Images were captured using the Leica THUNDER Imager Model Organism stereo microscope.
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