We synthesized nanocurcumin by solvent-antisolvent precipitation method following our optimized protocol. The curcumin powder (Alpha Chemika, Mumbai, India) was dissolved in acetone (10 mg/mL). One mL of the prepared solution was added to 15 mL deionized water (ultra-purified from Millipore Milli-Q system “resistivity 80 MΩ cm”) containing 0.5% w/v polyvinyl pyrrolidone (PVP; Sigma-Aldrich, St. Luis, MO, USA) in a drop-wise manner under stirring at 500 rpm for 1 min [25 (link)].
The synthesized curcumin nanoparticles were characterized by ultraviolet–visible (UV–Vis) spectrophotometry (Nanodrop, DeNovix, DS-11 FX + , US) to measure the absorption spectra between 200 and 800 nm. The mean particles’ size and the surface charge were measured by dynamic light scattering analysis using Zeta-seizer (Nano ZS, Malvern Instruments, Worcestershire, UK). Transmission electron microscope (TEM; JOEL, JSM-6360LA, JAPAN) examination of the nanoparticles was conducted to assess the shape, size, and distribution of the particles [25 (link), 26 (link)].
Nanocurcumin used at a minimum inhibitory concentration of 64 µg/mL against C. albicans ATCC90028 strain [27 (link)].
The synthesized curcumin nanoparticles were characterized by ultraviolet–visible (UV–Vis) spectrophotometry (Nanodrop, DeNovix, DS-11 FX + , US) to measure the absorption spectra between 200 and 800 nm. The mean particles’ size and the surface charge were measured by dynamic light scattering analysis using Zeta-seizer (Nano ZS, Malvern Instruments, Worcestershire, UK). Transmission electron microscope (TEM; JOEL, JSM-6360LA, JAPAN) examination of the nanoparticles was conducted to assess the shape, size, and distribution of the particles [25 (link), 26 (link)].
Nanocurcumin used at a minimum inhibitory concentration of 64 µg/mL against C. albicans ATCC90028 strain [27 (link)].