Angular resolved spectra were taken using a custom-built goniometer set-up [29 (link)]. Samples were prepared by cutting out a piece of roughly 1 cm2 out of the agar. This piece was subsequently attached to a microscope slide with double-sided tape. The slide containing the bacteria on agar was then mounted on a rotating stage, and illuminated using light in the UV–VIS region (Ocean Optics HPX-2000 xenon lamp) that was collimated through an optical fibre that was attached to a reflective collimator (Thorlabs RC08SMA-F01). This resulted in an illuminating light beam with a spot size of 5 mm diameter. The angle of incidence of this light could be varied by rotating the sample. Light that was reflected and scattered from the sample was collected using the same fibre–collimator combination as used for the incident beam. The fibre was subsequently connected to a spectrometer (AvaSpec-HS2048, Avantes). The detection fibre and collimator were mounted on a rotating arm so that the angle of detection could be varied. At the detection angle, which equals the negative of the incident angle, no signal was collected due to a limitation in the set-up. All the spectra reported here were normalized against a white diffuser (labsphere SRS-99-010) at 0° incident and 5° collection angle.
Hpx 2000 xenon lamp
Manufactured by OceanOptics
The HPX-2000 is a xenon lamp designed for use in laboratory equipment. It provides a broad-spectrum light source with a continuous output from 250 to 2500 nanometers. The lamp is powered by an external power supply and is intended for integration into other instruments or systems.
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Lab products found in correlation
2 protocols using hpx 2000 xenon lamp
1
Angular Resolved Spectroscopy of Bacterial Samples
2
Lens and Spectacle Transmittance in H. leopardinus
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We measured lens and spectacle transmittance of H. leopardinus (n = 2) using the methods described by Lind et al. (2013) (link) and Yovanovich et al. (2019) . The samples were placed on top of a black plastic disk with a 1-mm pinhole inside a custom-made matte black plastic cylinder (12 mm diameter × 10 mm height) with a circular (5 mm diameter) fused silica window in the bottom, filled with PBS. We used an HPX-2000 Xenon lamp (Ocean Optics, Dunedin, FL) to illuminate the samples via a 50-μm light guide (Ocean Optics) and collected transmitted light using a 1000-μm guide connected to a Maya2000 spectroradiometer controlled by SPECTRASUITE v.4.1 software (Ocean Optics). The guides were aligned with the container in a microbench system (LINOS, Munich, Germany). The reference measurement was taken from the container filled with PBS. We smoothed the curves using an 11-point running average and normalized to the highest value within the range 300–700 nm. From these data, we determined λT50 as the wavelength at which the light transmittance was 50% of the maximum.
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