All experimental procedures were approved by the Institutional Animal Care and Use Committee at New York University. Inbred BALB/cJ mice (Jackson Laboratories, 4 months old, female, n=7) were fixed by trans-cardiac perfusion of 4% paraformaldehyde in phosphate buffered saline (PBS) for ex vivo MRI. After fixation, mouse heads were removed and immersed in 4% PFA in PBS for 12 hours at 4°C before being transferred to PBS. The specimens were then kept in PBS (50 ml for each specimen) for 2 −3 weeks at 4°C to wash out residual fixatives, which reduce tissue T2 and the signal-to-noise ratio of the acquired images (37 (link)). Before ex vivo MRI, specimens were placed into 5 mL syringes, which were filled with Fomblin (Fomblin Profludropolyether, Ausimont, Thorofare, New Jersey, USA) for susceptibility matching and prevention of dehydration.
Inbred balb cj mice
Manufactured by Jackson ImmunoResearch
BALB/cJ mice are an inbred strain of laboratory mice. They are commonly used in a variety of research applications.
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Lab products found in correlation
2 protocols using inbred balb cj mice
1
Ex Vivo MRI of Fixed Mouse Brains
2
Ex Vivo MRI of Fixed Mouse Brains
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All experimental procedures were approved by the Institutional Animal Care and Use Committee at New York University. Inbred BALB/cJ mice (Jackson Laboratories, 4 months old, female, n=7) were fixed by trans-cardiac perfusion of 4% paraformaldehyde in phosphate buffered saline (PBS) for ex vivo MRI. After fixation, mouse heads were removed and immersed in 4% PFA in PBS for 12 hours at 4°C before being transferred to PBS. The specimens were then kept in PBS (50 ml for each specimen) for 2 -3 weeks at 4°C to wash out residual fixatives, which reduce tissue T 2 and the signal-to-noise ratio of the acquired images (37) . Before ex vivo MRI, specimens were placed into 5 mL syringes, which were filled with Fomblin (Fomblin Profludropolyether, Ausimont, Thorofare, New Jersey, USA) for susceptibility matching and prevention of dehydration.
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