The supernatant of lung lysates were used to determine the GSH concentration. In this method, the products of the reaction, thiols react with 2-dinitrobenzoicacid (DTNB) and form thiolate measurable at 412 nm. Therefore, the lung supernatant was added to a precipitating solution and then, was added to other solution containing 300 mM Na 2 HPO 4 and 0.05% DTNB. Later, this solution was measured using a Spectrophotometer Genesys 10uv (Thermo Fisher Scientific). The results obtained were corrected by protein and expressed as OD/mg protein (Beutler, Duron, & Kelly, 1963 ).
Spectrophotometer genesys 10uv
Manufactured by Thermo Fisher Scientific
The Spectrophotometer Genesys 10uv is a laboratory instrument designed to measure the absorbance or transmittance of light through a sample. It can be used to quantify the concentration of a substance in a solution by comparing the amount of light absorbed by the sample to a reference. The instrument covers a wavelength range from 190 to 1100 nanometers.
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Lab products found in correlation
2 protocols using spectrophotometer genesys 10uv
1
Glutathione Quantification in Lung Lysates
2
Catalase Activity Assay in Lung Lysates
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The supernatant of lung lysates were used to determine the CAT unit. Catalase activity was performed conforming to the Aebi (1984) protocol with minor modifications. The product of the reaction was measured after 1 min using Spectrophotometer Genesys 10uv (Thermo Fisher Scientific) at 240 nm. The results were normalized by protein and expressed as UCAT/mg protein.
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