Uplan 60x na 1.42 oil objective

Manufactured by Olympus

The UPlan 60X/NA 1.42 oil objective is a high-magnification lens designed for use in microscopy applications. It has a numerical aperture of 1.42 and is intended for use with oil immersion. The objective provides high-resolution imaging capabilities.

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Lab products found in correlation

Lsm 800, by Zeiss (1 mentions) Plan apochromat 20x 0.8 air objective, by Zeiss (1 mentions) Orca spark camera, by Hamamatsu Photonics (1 mentions) Orca er, by Hamamatsu Photonics (1 mentions) Cellsens dimension 2, by Olympus (1 mentions) Bx51 microscope, by Hamamatsu Photonics (1 mentions)

Spelling variants of this product

60X NA=1.42 Oil Objective (5 citations) 60× 1.42 NA objective (5 citations) 60x/1.42 oil objectives (2 citations)

2 protocols using uplan 60x na 1.42 oil objective

Confocal Imaging of Metaphase Spreads

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Confocal imaging was performed on an inverted Zeiss LSM 800 using the Zeiss Zen software, a Plan-Apochromat 20X/0.8 air objective with a pinhole size set at 1 Airy Unit (cell size measurement) or Plan-Apochromat 10X/0.45 air objective with a pinhole opened to the maximum diameter (phospho-histone H3 imaging for tail proliferation assays). For the metaphase spreads, images were acquired using Micromanager 1.4 software^{70 (link)} on an upright Olympus^® BX51 microscope equipped with an ORCA-ER and ORCA-Spark camera (Hamamatsu, Photonics) and Olympus UPlan 60X/NA 1.42 oil objective.

Cadart C., Bartz J., Oaks G., Liu M.Z, & Heald R. (2023). Polyploidy in Xenopus lowers metabolic rate by decreasing total cell surface area. Current biology : CB, 33(9), 1744-1752.e7.

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Chromosome and Nucleus Imaging Protocol

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Chromosomes were imaged using Micromanager 1.4 software⁷⁹ and nuclei were imaged using Olympus cellSens Dimension 2 software on an upright Olympus BX51 microscope equipped with an ORCA-ER or ORCA-Spark camera (Hamamatsu Photonics) and Olympus UPlan 60X/NA 1.42 oil objective. All images across all datasets were taken using the same exposure settings.

Kitaoka M., Smith O.K., Straight A.F, & Heald R. (2022). Molecular conflicts disrupting centromere maintenance contribute to Xenopus hybrid inviability. Current biology : CB, 32(18), 3939-3951.e6.

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