Cells isolated from LNs, FRCs or NIH/3T3 cell lines were suspended in FACS buffer (PBS 2% FCS, 2mM EDTA) and first blocked with anti-CD16/CD32 (eBioscience) for all staining procedures. Cells were counted on a FACS Calibur by reference to fluorescence beads. Stained cells were analysed on either a FACS Calibur or LSRII. Antibodies used for staining: CD45.1 (BD Biosciences), CD140a (clone APA5 eBioscience), CD31 (BD Biosciences), PDPN (clone 8.1.1 eBioscience), CD35 (clone 8C12 BD Biosciences), VCAM-1 (clone M/K-2 Abcam), CD44 (clone IM7 BD Biosciences), CD3 (BD Biosciences), CD19 (clone ID3 BD Biosciences), MHCII (I-A/I-E BD Biosciences), CD11c (clone HL3 BD Biosciences).
Mhcii 1 a 1 e
Manufactured by BD
MHCII (I-A/I-E) is a lab equipment product offered by BD. It is used to detect and analyze major histocompatibility complex class II proteins, which play a crucial role in the immune system's antigen presentation process. The product's core function is to provide researchers with the necessary tools to study these important proteins, without making any claims about its intended use or applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd140a clone apa5, by Thermo Fisher Scientific (2 mentions)
Pdpn clone 8, by Thermo Fisher Scientific (2 mentions)
Cd35 clone 8c12, by BD (2 mentions)
Vcam 1 clone m k 2, by Abcam (2 mentions)
Cd19 clone id3, by BD (2 mentions)
Cd44 clone im7, by BD (2 mentions)
Anti cd16 cd32, by Thermo Fisher Scientific (2 mentions)
Cd11c clone hl3, by BD (2 mentions)
2 protocols using mhcii 1 a 1 e
1
Phenotypic Characterization of Lymph Node Cells
2
Phenotypic Characterization of Lymph Node Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells isolated from LNs, FRCs or NIH/3T3 cell lines were suspended in FACS buffer (PBS 2% FCS, 2mM EDTA) and first blocked with anti-CD16/CD32 (eBioscience) for all staining procedures. Cells were counted on a FACS Calibur by reference to fluorescence beads. Stained cells were analysed on either a FACS Calibur or LSRII. Antibodies used for staining: CD45.1 (BD Biosciences), CD140a (clone APA5 eBioscience), CD31 (BD Biosciences), PDPN (clone 8.1.1 eBioscience), CD35 (clone 8C12 BD Biosciences), VCAM-1 (clone M/K-2 Abcam), CD44 (clone IM7 BD Biosciences), CD3 (BD Biosciences), CD19 (clone ID3 BD Biosciences), MHCII (I-A/I-E BD Biosciences), CD11c (clone HL3 BD Biosciences).
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