Mouse anti human met

Manufactured by Cell Signaling Technology

The Mouse anti-human MET is a laboratory reagent used in research applications. It is an antibody that specifically binds to the MET protein, which is a receptor tyrosine kinase involved in cellular signaling pathways. This product can be used in various experimental techniques, such as Western blotting and immunohistochemistry, to detect and analyze the expression and localization of the MET protein in biological samples.

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Lab products found in correlation

Ab66400, by Abcam (1 mentions) Ab76055, by Abcam (1 mentions) Mab1880, by R&D Systems (1 mentions) Mab4165, by R&D Systems (1 mentions) Mab9601, by R&D Systems (1 mentions) Hrp conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Goat anti mouse igg hrp, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg hrp, by Cell Signaling Technology (1 mentions) Rabbit anti human egfr, by Cell Signaling Technology (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)

2 protocols using mouse anti human met

Antibody Profiling of Extracellular and Intracellular Markers

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Antibodies
used in this study are as follows.
Anti-extracellular part: mouse anti-human CD9 (MAB1880, R&D),
rabbit anti-human CD63 (MAB5048, R&D), mouse anti-human CD41b
(555468, BD), mouse anti-human CD81 (MAB4165, R&D), mouse anti-human
CD82 (MAB4616, R&D), mouse anti-human E-cadherin (AB8993, Abcam),
and mouse anti-human EpCAM (MAB9601, R&D). Anti-intracellular
part: rabbit anti-human CD9 N-term (AP1482a, Abgent), rabbit anti-human
CD81 C-term (AP6631b, Abgent), anti-CD82 C-term (AB66400, Abcam),
and mouse anti-human E-cadherin C-term (AB76055, Abcam). Anti-mouse
IgG (557273, BD Biosciences), mouse anti-human MET (5631, Cell Signaling),
and HRP-conjugated secondary antibodies (Jackson Immunoresearch) were
also used.

Zhu L., Wang K., Cui J., Liu H., Bu X., Ma H., Wang W., Gong H., Lausted C., Hood L., Yang G, & Hu Z. (2014). Label-Free Quantitative Detection of Tumor-Derived Exosomes through Surface Plasmon Resonance Imaging. Analytical Chemistry, 86(17), 8857-8864.

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EGFR and c-MET Expression Validation

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EGFR and c-MET expression in cell lines was validated by western blotting using their respective primary antibodies (rabbit anti-human EGFR and mouse anti-human MET; Cell Signaling Technology, 1:1000). Secondary antibodies (goat anti-rabbit IgG-HRP and goat anti-mouse IgG-HRP; Cell Signaling Technology, 1:3000) followed by the SuperSignal® West Pico Chemiluminescent Substrate (Thermo-Scientific) were applied. Bands corresponding to each receptor were quantified based on pixel intensity and normalized to those from the β-actin control using G:Box 9 (Syngene). Detailed procedures are described in the Supplementary Material.

Cavaliere A., Sun S., Lee S., Bodner J., Li Z., Huang Y., Moores S.L, & Marquez-Nostra B. (2020). Development of 89Zr-Amivantamab bispecific to EGFR and c-MET for PET imaging of triple-negative breast cancer. European journal of nuclear medicine and molecular imaging, 48(2), 383-394.

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