The PBMCs were suspended at a concentration of 106 cells per mL in RPMI-1640 supplemented with 1% penicillin/streptomycin (Sigma-Aldrich, Germany), 2 mM L-glutamate (Gibco, USA), and 10% heat-inactivated fetal bovine serum (Gibco, USA) in 48-well sterile (non-pyrogenic) polystyrene flat bottom plates (Corning, USA) in the presence or absence of 5 μg/mL phytohemaglutinin (PHA; Sigma-Aldrich, Germany). Cells were cultured at 37°C in humidified atmosphere of 5% CO2. The cell-free supernatants were collected 72 h later and frozen in aliquots at −80°C.
Phytohemaglutinin pha
Manufactured by Merck Group
Sourced in Germany, United States
Phytohemaglutinin (PHA) is a lectin derived from the red kidney bean (Phaseolus vulgaris). PHA has the ability to agglutinate human red blood cells, leading to its use as a tool in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
L glutamate, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Phenol red, by Sartorius (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Hepes, by Sartorius (1 mentions)
Lc ms grade solvents, by Merck Group (1 mentions)
2 protocols using phytohemaglutinin pha
1
PBMC Culture and Stimulation Protocol
2
Cytotoxicity and Immune Assay Reagents
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Analytical grade solvents were from Sintorgan Labs. (Buenos Aires, Argentina); LC-MS grade solvents were from Merck (Darmstadt, Germany); 3-[4.5-dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide (MTT), Histopaque 1077, phytohemaglutinin (PHA) and lipopolysaccharides (LPS), were from Sigma-Aldrich (Misuri, USA.); ursolic acid was from Indofine (Nueva Jersey, USA); penicillin/streptomycin/amphotericin B (100X), phosphate buffered saline (PBS) and Hank's salt (HBSS) for cell culture, were from MicroVet Labs. (Buenos Aires, Argentina); Trypan blue was from Cicarelli Labs. (Santa Fé, Argentina); fetal bovine serum (FBS) was from Natocor Biotechnology (Córdoba, Argentina). RPMI 1640 with L-Glutamine, HEPES and phenol red (serum-free RPMI 1640 medium) was from Biological Industries (Cromwell, USA).
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