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Taqman one step pcr master mix reagents kit

Manufactured by Thermo Fisher Scientific
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The TaqMan One Step PCR Master Mix Reagents Kit is a ready-to-use solution for performing one-step reverse transcription and quantitative PCR (RT-qPCR) reactions. The kit includes all the necessary components, including a proprietary enzyme blend, buffers, and dNTPs, to facilitate the detection and quantification of RNA targets.

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TaqMan Master Mix (448 citations) PCR Master Mix (310 citations) TaqMan reagents (162 citations) TaqMan PCR kit (42 citations) TaqMan kits (18 citations) Taqman master mix reagent (7 citations) Taqman PCR reagents (2 citations) Step One PCR (2 citations)

2 protocols using taqman one step pcr master mix reagents kit

1

qRT-PCR Analysis of HPSE and p53 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the qRT-PCR, 100 ng of total RNA was used to prepare cDNA (TaqMan One Step PCR Master Mix Reagents Kit) (Applied Biosystem, Carlsbad, CA). Specific primers and probes that were designed, tested, and standardized by Applied Biosystems for HPSE and p53 were used (HPSE: Hs00935036_m1; p53: Hs01034249_m1). The difference in cycle threshold values (ΔCT) of HPSE was normalized to the ΔCT of GAPDH (Glyceraldehude-3-phospate dehydrogenase, Hs99999905_m1), and the fold-change in expression was expressed relative to CD14+ monocytes, considered as a positive control while mesemchymal stem cells were used as a negative control. For p53 mRNA quantification the difference in cycle threshold values (ΔCT) of p53 was normalized to the ΔCT of GAPDH (Glyceraldehyde-3-phospate dehydrogenase, Hs99999905_m1), and the fold-change in expression was expressed relative to FI-T.
Caruana I., Savoldo B., Hoyos V., Weber G., Liu H., Kim E.S., Ittmann M.M., Marchetti D, & Dotti G. (2015). Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T-lymphocytes. Nature medicine, 21(5), 524-529.
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2

qRT-PCR Analysis of HPSE and p53 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the qRT-PCR, 100 ng of total RNA was used to prepare cDNA (TaqMan One Step PCR Master Mix Reagents Kit) (Applied Biosystem, Carlsbad, CA). Specific primers and probes that were designed, tested, and standardized by Applied Biosystems for HPSE and p53 were used (HPSE: Hs00935036_m1; p53: Hs01034249_m1). The difference in cycle threshold values (ΔCT) of HPSE was normalized to the ΔCT of GAPDH (Glyceraldehude-3-phospate dehydrogenase, Hs99999905_m1), and the fold-change in expression was expressed relative to CD14+ monocytes, considered as a positive control while mesemchymal stem cells were used as a negative control. For p53 mRNA quantification the difference in cycle threshold values (ΔCT) of p53 was normalized to the ΔCT of GAPDH (Glyceraldehyde-3-phospate dehydrogenase, Hs99999905_m1), and the fold-change in expression was expressed relative to FI-T.
Caruana I., Savoldo B., Hoyos V., Weber G., Liu H., Kim E.S., Ittmann M.M., Marchetti D, & Dotti G. (2015). Heparanase promotes tumor infiltration and antitumor activity of CAR-redirected T-lymphocytes. Nature medicine, 21(5), 524-529.
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