Soybean lox

Manufactured by Merck Group

Sourced in United States

Soybean LOX is a lab equipment product that functions as a lipoxygenase enzyme. Lipoxygenases are non-heme iron-containing dioxygenases that catalyze the incorporation of molecular oxygen into polyunsaturated fatty acids.

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Lab products found in correlation

Trolox, by Merck Group (2 mentions) Linoleic acid sodium salt, by Merck Group (2 mentions) Nordihydroguaiaretic acid ndga, by Merck Group (1 mentions) α glucosidase from saccharomyces cerevisiae, by Merck Group (1 mentions) Mushroom tyrosinase, by Merck Group (1 mentions)

Close spelling variants of this product

Soybean LOX-1

4 protocols using soybean lox

Evaluating Antioxidant Activities of Nitrones

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NDGA, Trolox, AAPH, soybean LOX, and linoleic acid sodium salt were purchased from Aldrich Chemical Co. (Milwaukee, WI, USA). Phosphate buffer (0.1 M, pH 7.4) was prepared by mixing an aqueous KH₂PO₄ solution (50 mL, 0.2 M), and an aqueous NaOH solution (78 mL, 0.1 M); 2-Amino-2-hydroxymethyl-propane-1,3-diol (Tris) was used as a buffer pH 9. A lambda 20 (Perkin–Elmer-PharmaSpec 1700, Perkin-Elmer Corporation Ltd., Lane Beaconsfield, Bucks, UK) UV–Vis double beam spectrophotometer was used for the assays.
To measure in vitro antioxidant activity of the nitrones 8 and 9, the following assays were used: The inhibition of lipid peroxidation (LP) induced by AAPH, the DMSO method for hydroxyl radical scavenging activity, the ABTS^+·–decolorization assay and the in vitro inhibition of soybean lipoxygenase (LOX).

Hadjipavlou-Litina D., Głowacka I.E., Marco-Contelles J, & Piotrowska D.G. (2022). Synthesis and Antioxidant Properties of Novel 1,2,3-Triazole-Containing Nitrones. Antioxidants, 12(1), 36.

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Antioxidant and Anti-inflammatory Evaluation

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Several assays were used to evaluate in vitro antioxidant activity of nitrones, such as the inhibition of lipid peroxidation (LP), induced by AAPH in the presence of atmospheric oxygen, competition of the tested compounds with DMSO, in terms of hydroxyl radical scavenging activity, and ABTS^+∙ decolorization assay. In vitro inhibition of soybean lipoxygenase (LOX) was used to determine anti-inflammatory activity. Reagents and materials: nordihydroguaiaretic acid (NDGA), Trolox, 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), soybean LOX, and linoleic acid sodium salt were from Aldrich Chemical Co. Milwaukee, WI, (USA). Phosphate buffer (0.1 M, pH 7.4) was prepared by mixing an aqueous KH₂PO₄ solution (50 mL, 0.2 M), and an aqueous NaOH solution (78 mL, 0.1 M); pH (7.4) was adjusted by adding a solution of KH₂PO₄ or NaOH. A Lambda 20 (Perkin–Elmer-PharmaSpec 1700, Boston, MA, USA) UV–Vis double beam spectrophotometer was used for the assays.

Diez-Iriepa D., Knez D., Gobec S., Iriepa I., de los Ríos C., Bravo I., López-Muñoz F., Marco-Contelles J, & Hadjipavlou-Litina D. (2022). Polyfunctionalized α-Phenyl-tert-butyl(benzyl)nitrones: Multifunctional Antioxidants for Stroke Treatment. Antioxidants, 11(9), 1735.

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Antioxidant Properties of M. lupulina

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Flowering above-ground parts of M. lupulina were collected near Lake Jarun, in the surroundings of Zagreb (45°48′ N 15°90′ E). The producer of PPG 425 was A&C (Clonmel, Ireland). Purity of standards (acarbose, BHA, diclofenac, EDTA, kojic acid, nordihydroguaiaretic acid (NDGA) and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox)) was ≥ 98.5%. The standards and the enzymes (α-glucosidase from Saccharomyces cerevisiae, α-amylase and elastase from porcine pancreas, mushroom tyrosinase and soybean LOX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Other reagents and chemicals were of analytical grade. The plant material was identified by Vedran Šegota, expert associate of Herbarium Croaticum at Division of Botany, Department of Biology, Faculty of Science, University of Zagreb. Voucher specimen is deposited in the Department of Pharmacognosy, University of Zagreb Faculty of Pharmacy and Biochemistry, Zagreb, Croatia (FG-2019-ML).

Jakupović L., Kalvarešin M., Bukovina K., Poljak V., Vujić L, & Zovko Končić M. (2021). Optimization of Two Eco-Friendly Extractions of Black Medick (Medicago lupulina L.) Phenols and Their Antioxidant, Cosmeceutical, α-Glucosidase and α-Amylase Inhibitory Properties. Molecules, 26(6), 1610.

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Enzymatic Synthesis and Analysis of Oxylipins

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(13S)-HPOT was prepared from 0.5 mM linolenic acid (LnA) using 7 μg soybean LOX (L7395, Sigma-Aldrich) in 1 mL 50 mM sodium phosphate buffer (pH 7.0) for 10 min at room temperature. An assay of the coupled OsAOC-OsAOS1 reaction was conducted as follows. OsAOS1 and OsAOC(I) (total 5 μg combined) were mixed to achieve molar ratios from 1:0 to 1:20 and added to (13S)-HPOT prepared by soybean LOX, to reach a total reaction volume of 2 mL. Reaction products were extracted. cis-OPDA was separated by straight phase HPLC (SP-HPLC), and the stereochemistry of cis-OPDA was analyzed by chiral phase HPLC (CP-HPLC) as described previously (Yoeun et al., 2013 (link)). Alternatively, the reaction mixture was injection directly, without extraction onto reverse phase HPLC (RP-HPLC), and HPLC was developed in methanol: water: acetic acid (80:20:0.01). All HPLC analyses were performed with UV detection at 205, 220, and 234 nm for α-ketol, OPDA, and HPOT, respectively.

Yoeun S., Cho K, & Han O. (2018). Structural Evidence for the Substrate Channeling of Rice Allene Oxide Cyclase in Biologically Analogous Nazarov Reaction. Frontiers in Chemistry, 6, 500.

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