Polarstar omega microplate reader

Manufactured by BMG Labtech

Sourced in Germany, United States, Australia

The POLARstar Omega is a multi-mode microplate reader designed for diverse applications. It measures absorbance, fluorescence, and luminescence in 96- and 384-well microplates. The POLARstar Omega provides accurate and reliable data for a wide range of life science research and drug discovery assays.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (2 mentions) Methyl thiazolyl tetrazolium (mtt), by BMG Labtech (2 mentions) Synergie h4 hybrid reader, by Agilent Technologies (2 mentions) Picogreen dye, by Thermo Fisher Scientific (2 mentions) Picogreen, by BMG Labtech (2 mentions) Methyl thiazolyl tetrazolium (mtt), by American Type Culture Collection (2 mentions) 384 well flat clear bottom microplate, by Corning (2 mentions) Dual luciferase reporter assay system, by Promega (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Alamarblue cell viability assay kit, by Thermo Fisher Scientific (2 mentions) Celltiter glo luminescent cell viability assay, by Promega (2 mentions) Alamar blue, by Thermo Fisher Scientific (2 mentions) Itraconazole, by Corning (2 mentions) Itraconazole, by Merck Group (2 mentions) Lapatinib, by Merck Group (2 mentions) Lapatinib, by Cayman Chemical (2 mentions) Blyscan glycosaminoglycan assay, by Biocolor (2 mentions) Model 680xr microplate reader, by Bio-Rad (2 mentions) Papain, by Merck Group (2 mentions) Synergy h4 microplate reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

POLARstar Omega (453 citations) Microplate reader (315 citations) POLARstar microplate reader (23 citations) Omega microplate reader (15 citations) POLARstar Omega multi-mode microplate reader (6 citations) POLARstar Omega reader (5 citations) POLARstar Omega multidetection microplate reader (3 citations) POLARstar Omega Multi-Mode Microplate Reader with Fluorescent Polarization (2 citations) POLARstar® Omega multifunction microplate reader (2 citations)

110 protocols using polarstar omega microplate reader

Fluorescence-based Bacterial Growth Assay

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Azidobenzyl-luciferin 9 was serially diluted two-fold in PBS and 50 μL of each concentration was added to 50 μL minimal media containing 2 μM of BCN-biotin 1 probe, resulting in a starting concentration of 20 μM azidobenzyl-luciferin. After incubation at 37 °C for 3 h, 100 μL MeCN containing 2 μM of tetrazine-BDP-FL 2 was added and fluorescence was determined at an excitation wavelength of 485 nm and emission at 520 nm (POLARstar Omega Microplate reader, BMG Labtech). The same procedure was repeated with azido-cefoxitin 10, with a starting concentration of 400 μM.
To study the effect of azidobenzyl-luciferin 9 on both ΔTolC E. coli strains, overnight cultures grown in minimal media were diluted to OD₆₀₀ 0.05 and allowed to grow to mid-log phase. The diluted bacterial cultures were then incubated at 37 °C with 5 and 50 μM azidobenzyl-luciferin 9 for 0, 3, 6, 18 h. The absorbance reading at each timepoint was recorded using a plate reader at 600 nm (POLARstar Omega Microplate reader, BMG Labtech). This procedure was repeated for the assessment of azido-cefoxitin 10 (75, 150, and 750 μM) toxicity.

Ooi J.M., Fairhall J.M., Spangler B., Chong D.J., Feng B.Y., Gamble A.B, & Hook S. (2022). Development of a bioorthogonal fluorescence-based assay for assessing drug uptake and delivery in bacteria. RSC Advances, 12(25), 15631-15642.

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MTT Assay for Cell Viability

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The MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to assess RT112 cell viability. After the cells were seeded and cultured overnight, they were treated with bacterial supernatants for one to three days. At the end of the experiment, they were incubated in 0.45 mg/mL MTT (Life Technologies) at 37°C for 30 minutes. The absorbance at 595 nm of MTT-formazan was detected spectrophotometrically using an POLARstar Omega Microplate Readers (BMG Labtech). The percentage of cell viability was determined by normalising the absorbance value in each condition to the mock control.

Then C.K., Paillas S., Moomin A., Misheva M.D., Moir R.A., Hay S.M., Bremner D., Roberts (nee Nellany) K.S., Smith E.E., Heidari Z., Sescu D., Wang X., Suárez-Bonnet A., Hay N., Murdoch S.L., Saito R., Collie-Duguid E.S., Richardson S., Priestnall S.L., Wilson J.M., Gurumurthy M., Royle J.S., Samuel L.M., Ramsay G., Vallis K.A., Foster K.R., McCullagh J.S, & Kiltie A.E. (2024). Dietary fibre supplementation enhances radiotherapy tumour control and alleviates intestinal radiation toxicity. Microbiome, 12, 89.

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MTT Assay for Cell Viability

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MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was applied to assess cell viability by adding 0.25 mg/mL MTT (Life Technologies) to cells at 37 °C for 1 h in the end of experiment. The absorbance at 595 nm of MTT-formazan was detected spectrophotometrically using POLARstar Omega Microplate Readers (BMG Labtech) after dissolution of the crystals in isopropanol. The percentage of cell viability was calculated by the formula: [Experimental group/Control group] × 100%.

Then C.K., Paillas S., Wang X., Hampson A, & Kiltie A.E. (2020). Association of Bacteroides acidifaciens relative abundance with high-fibre diet-associated radiosensitisation. BMC Biology, 18, 102.

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Intracellular ROS Measurement in VHSV-Exposed RBCs

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The intracellular ROS level was assessed in VHSV-exposed RBCs using the cell-permeant 2',7'-dichlorodihydrofluorescein diacetate (DCFDA, Sigma-Aldrich). RBCs were exposed to VHSV at MOI 1, during 72 h, at 14°C. After that, RBCs were washed with RPMI and incubated with 20 μM DCFDA in RPMI, for 30 min at RT. Fluorescence intensity of 2′,7′-dichlorofluorescin was measured using POLARstar Omega microplate reader (BMG LABTECH, USA) at excitation 480 nm and emission 530 nm.

Nombela I., Puente-Marin S., Chico V., Villena A.J., Carracedo B., Ciordia S., Mena M.C., Mercado L., Perez L., Coll J., Estepa A, & Ortega-Villaizan M.D. (2018). Identification of diverse defense mechanisms in rainbow trout red blood cells in response to halted replication of VHS virus. F1000Research, 6, 1958.

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Amyloid Formation Kinetics by ThT

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The fluorescent amyloid reporter dye Thioflavin T was used to follow the kinetics of amyloid formation. Amyloid assembly was initiated by diluting proteins to a final protein concentration of 2.5 μM and urea concentration of 300 mM with 25 mM NaH₂PO₄, 150 mM NaCl, 40 μM ThT, 0.5 mM DTT, pH 7.4. Assays were conducted in triplicate, at 37°C in Costar black 96-well fluorescence plates (Corning) in a POLARstar Omega microplate reader (BMG Labtech) with fluorescence excitation at 440 nm and emission at 480 nm.

Steain M., Baker M.O., Pham C.L., Shanmugam N., Gambin Y., Sierecki E., McSharry B.P., Avdic S., Slobedman B., Sunde M, & Abendroth A. (2020). Varicella zoster virus encodes a viral decoy RHIM to inhibit cell death. PLoS Pathogens, 16(7), e1008473.

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Measurement of Cellular Oxidative Stress

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ROS generation was assessed using the H₂DCFDA and MitoSOX Red dyes, allowing the detection of cellular hydrogen peroxide and mitochondrial superoxide anion, respectively [21 (link), 22 (link)]. Cells were washed with warm HBSS and then incubated for 25 min at 37°C with 2 μM H₂DCFDA or 5 μM MitoSOX Red, respectively, diluted in HBSS. Cells were then gently washed once with warm HBSS buffer. Fluorescence intensity of living cells was determined by spectrofluorimetry using the POLARstar Omega microplate reader (BMG Labtech) with excitation/emission wavelengths of 485/520 nm for H₂DCFDA and 520/590 nm for MitoSOX Red. Fluorescence intensity values were normalized to the total protein content. Results were expressed in comparison to nonsteatotic or steatotic untreated cells, as appropriate.

Bucher S., Le Guillou D., Allard J., Pinon G., Begriche K., Tête A., Sergent O., Lagadic-Gossmann D, & Fromenty B. (2018). Possible Involvement of Mitochondrial Dysfunction and Oxidative Stress in a Cellular Model of NAFLD Progression Induced by Benzo[a]pyrene/Ethanol CoExposure. Oxidative Medicine and Cellular Longevity, 2018, 4396403.

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Antimicrobial Peptide Potency Assay

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The MICs were measured using a liquid growth inhibition assay in poor broth (PB: 1% bactotryptone, 0.5% NaCl w/v). 10 μL of serial dilutions of peptides (MccJ25, MccJ25[His5Lys], MccJ25[His5Ala], Cap) were added to 90 μL of a mid-logarithmic growth phase culture of E. coli W3110 diluted in PB to an optical density of 0.001 at 600 nm. Each peptide dilution was tested in triplicate. The plates were incubated at 37°C under shaking in a POLARstar OMEGA microplate reader (BMG Labtech), and the bacterial growth was monitored over 120 min by measurement of the optical density at 600 nm. The MICs were determined as the lowest peptide concentrations showing complete inhibition of the bacterial growth.

Mathavan I., Zirah S., Mehmood S., Choudhury H.G., Goulard C., Li Y., Robinson C.V., Rebuffat S, & Beis K. (2014). Structural basis for hijacking siderophore receptors by antimicrobial lasso peptides. Nature chemical biology, 10(5), 340-342.

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Luminescence-based O-GlcNAcylation Assay

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This assay evaluates O-GlcNAcylation through monitoring UDP formation in glycosyltransferase reactions by luminescence. Briefly, OGT reactions were carried out in a 50-μL final volume, containing 0.1 mM UDP-GlcNAc, 200 nM purified full-length OGT, 100 μM RBL-2 peptide in OGT reaction buffer (25 mM Tris-HCl, pH 7.5; 1 mM DTT; 12.5 mM MgCl₂). Reactions were incubated at 37 °C for 2 h. Afterwards, each reaction was transferred in duplicate into a 96-well white microplate and was mixed with a 1:1 ratio of the UDP-Glo Detection Reagent. After incubation at room temperature for 1 h, the luminescence was recorded with a POLARstar^® Omega microplate reader (BMG LABTECH) or with a BioTek Synergy™ H4 microplate reader. The data were plotted with GraphPad prism software, version 8, [Inhibitor] vs. response-variable slope.

Loi E.M., Weiss M., Pajk S., Gobec M., Tomašič T., Pieters R.J, & Anderluh M. (2020). Intracellular Hydrolysis of Small-Molecule O-Linked N-Acetylglucosamine Transferase Inhibitors Differs among Cells and Is Not Required for Its Inhibition. Molecules, 25(15), 3381.

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Assessing U87 Cell Viability with CCK-8

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The Cell viability of U87 cells was determined using CCK-8 assay (Dojindo). U87 cells at the density of 1 × 10⁴ cells per well/100 μL were seeded in 96-well plate and cultured for 24 h to allow adherence and growth. After this time, cells were incubated with synthetic peptide using 0, 5, 10 μM doses for 4 h. After the treatment, cells were exposed to CCK-8 according to the manufacturer's instructions. The absorbance per well was measured at 540 nm using the POLARstar Omega Microplate Reader (BMG Labtech). Relative cell survival was expressed relative to the non-treated controls.

Teng Y., Liu S., Guo X., Liu S., Jin Y., He T., Bi D., Zhang P., Lin B., An X., Feng D., Mi Z, & Tong Y. (2017). An Integrative Analysis Reveals a Central Role of P53 Activation via MDM2 in Zika Virus Infection Induced Cell Death. Frontiers in Cellular and Infection Microbiology, 7, 327.

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Monitoring Hydrophobin Rodlet Formation

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Thioflavin-T (ThT) fluorescence assays were used to monitor rodlet formation by hydrophobins. Rodlets, but not soluble hydrophobins, have an underlying cross-β structure which binds the amyloid-specific dye ThT and results in increased fluorescence. Rodlet formation was induced by double orbital shaking at 300–700 rpm and at 25–50 °C in a Costar 96-well fluorescence plate (Corning) inside a POLARstar Omega microplate reader (BMG Labtech). Unless otherwise stated, protein solutions (100 μL, 25 μg/mL) were prepared in 25 mM NaH₂PO₄, pH 7.2 containing 20–40 μM ThT in the presence or absence of additives. For pH assays, experiments were performed in 20 mM Tris or sodium acetate to achieve a pH of 3 or 7, respectively. The exact condition (including additives) used is detailed in the legend or results section. Samples in triplicate were excited at 440 nm and fluorescence emission was measured at 480 nm every 60 s.

Pham C.L., Rey A., Lo V., Soulès M., Ren Q., Meisl G., Knowles T.P., Kwan A.H, & Sunde M. (2016). Self-assembly of MPG1, a hydrophobin protein from the rice blast fungus that forms functional amyloid coatings, occurs by a surface-driven mechanism. Scientific Reports, 6, 25288.

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