Het 1a

HNSCC cell lines UMSCC-1, UMSCC-10A, UMSCC22B, Cal33, UPCI 4B, UPCI 15B, 1483 and 686LN were obtained from Dr. Jennifer R. grandis (University of California, San Francisco, CA) as described previously [19 (link)]. Het-1A (CRL-2692), a human esophageal squamous epithelial cell line, was obtained from ATCC (Manassas, VA) and cultured in Airway Epithelial Cell Basal Growth Medium with supplement mix (Promo Cell, Heidelberg, Germany). UMSCC-1, Cal33, UMSCC-10A, UMSCC22B, UPCI 4B, UPCI 15B and 1483 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Fisher Scientific, Pittsburgh, PA) supplemented with 10% fetal bovine serum (FBS) (Invitrogen, Carlsbad, CA), penicillin (100 U/ml)/streptomycin (100 μg/ml) at 37 °C in a humidified atmosphere of 5% CO₂. OSC19 cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Fisher) plus 10% FBS and Non-Essential Amino Acid (Fisher). 686LN cells were maintained in Ham’s F-12 medium (Fisher) containing 10% FBS, 100 units/ml penicillin, and 100 μg/ml streptomycin. All cell lines were authenticated by the Research Animal Diagnostic Laboratory by species-specific PCR testing within 6 months of use.

HET-1A (CRL-2692) and SCC-15 (CRL-1623) cell lines were purchased from ATCC (American Type Culture Collection, Wesel, Germany) and were cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with 400 ng/ml hydrocortisone (H4001, Thermo Fisher Scientific, MA, USA), 10% fetal bovine serum (FBS, 10270106, Thermo Fisher Scientific, MA, USA), 100 U/ml penicillin and 100 µg/ml streptomycin at 37°C with 5% CO₂.

Four human ESCC cell lines (Eca109, EC9706, KYSE150, KYSE450) and human normal esophageal epithelial cell line (Het-1A) were all procured from the ATCC (Manassas, VA, USA) and propagated at 37 °C in a humidified incubator of 5% CO₂. Cell lines were cultured in RPMI-1640 medium (Gibco, Carlsbad, CA, USA) with 10% FBS (Gibco) and 1% Pen/Strep mixture.