MDA-MB-231 highly invasive breast cancer cells and MCF-7 epithelial cancer cells were acquired from the American Type Culture Collection (ATCC, USA). Both cell lines are derived from breast adenocarcinoma, with MCF-7 cells retaining some characteristics of the differentiated mammary epithelium.All cell lines were incubated in phenol red-free DMEM culture medium with 10% fetal bovine serum, 2 mM L-Glutamine, and 100 U/mL penicillin and 100 µg/mL streptomycin at 37 •C and 5% CO2. Cells were passaged every 3-5 days, no more than 15 times. All solutions, buffers and media used for cell culture were purchased from PAN-Biotech (Aidenbach, Germany).
Mda mb 231 highly invasive breast cancer cells
Manufactured by American Type Culture Collection
Sourced in United States, Poland
MDA-MB-231 is a highly invasive breast cancer cell line derived from a pleural effusion of a 51-year-old Caucasian female with metastatic breast adenocarcinoma. These cells exhibit a mesenchymal-like morphology and are commonly used in cancer research to study tumor invasion and metastasis.
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Breast Cancer Cell Culture Protocols
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Breast Cancer Cell Culturing Protocol
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MDA-MB-231 highly invasive breast cancer cells and MCF-7 epithelial cancer cells were acquired from the American Type Culture Collection (ATCC, Kielpin Lomianki, Poland). Both cell lines are derived from breast adenocarcinoma, with MCF-7 cells retaining some characteristics of the differentiated mammary epithelium. All cell lines were incubated in phenol red-free DMEM culture medium with 10% fetal bovine serum, 2 mM L-glutamine, and 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C and 5% CO2. Cells were passaged every 3–5 days, no more than 15 times. All solutions, buffers, and media used for cell culture were purchased from PAN-Biotech (Aidenbach, Germany).
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