Bcl 2 sirna

Chitosan (CS, 200–500 kDa) was purchased from Charming & Beauty Co., Ltd. (Taipei, Taiwan). Sodium hyaluronic acid (HA, 310 kDa) was purchased from Carelife Technology Corp. (Taipei, Taiwan). 2-Aminoethanethiol (cysteamine) and 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Acros Organics (Geel, Belgium). Sodium hydroxide, monochloroacetic acid, disodium hydrogen phosphate heptahydrate, calcium chloride, isopropyl alcohol, dopamine hydrochloride (Dopa), N-hydroxysulfosuccinimide sodium (NHS), doxorubicin hydrochloride (Dox), HEPES, phosphate-buffered saline (PBS), glutathione (GSH) and Hoechst 33342 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Negative control siRNA (siNC, sense strand: 5′-UUC UCC GAA CGU GUC ACG UTT-3′, anti-sense: 5′-ACG UGA CAC GUU CGG AGA ATT-3′), FAM-labeled negative control siRNA (FAM-siRNA) and Bcl-2 siRNA (siBcl-2, sense: 5′-GUA CAU CCA UUA UAA GCU GdTdT-3′, anti-sense: 5′-CAG CUU AUA AUG GAU GUA C-dTdT-3′) were purchased from GenePharma Co., Ltd. (Shanghai, China). Nuclease-free water and 5,5′-dithio-bis-(2-nitrobenzoic acid) (Ellman’s reagent) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A Qubit™ RNA BR assay kit was purchased from Invitrogen (Waltham, MA, USA). Cell Proliferation Reagent WST-1 was purchased from Roche (Basel, Switzerland).