Total rna extraction kit

Apigenin (purity ≥95%), OVA (grade V), aluminum hydroxide, and histamine
dihydrochloride were purchased from Sigma-Aldrich Co, St Louis, Missouri.
Montelukast was obtained from Cipla Limited, Mumbai, India. Mouse OVA-specific
IgE, total IgE, total IgG1, β-hexosaminidase, IL-4, IL-5, IL-13, IL-17, IFN-γ,
and Leukotriene C4 enzyme-linked immunosorbent assay (ELISA) kit were obtained
from Bethyl Laboratories Inc, Montgomery, Texas. The primary antibodies of
GATA3, T-bet, NF-κB, IκBα, p-STAT6, suppressor of cytokine signaling 1 (SOCS1),
and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were purchased from Abcam,
Cambridge, Massachusetts. Total RNA extraction kit and real time-polymerase
chain reaction (RT-PCR) kit were purchased from MP Biomedicals India Private
Limited, India.

Streptozotocin and berberine hydrochloride hydrate (purity 99%) were purchased
from Sigma Chemical Co (St Louis, Missouri). Insulin injection (Mixtard) was
purchased from Novo Nordisk India Limited, India. Cholesterol and triglyceride
kit were obtained from Accurex Biomedical Pvt Ltd, India. Further, TNF-α, IL-1β,
IL-6, and BDNF enzyme-linked immunosorbent assay (ELISA) kits were obtained from
Thermo Scientific (Chengdu, SiChuan). Insulin obtained from Mercodia AB,
Uppsala, Sweden, and glycated hemoglobin (Hb) assay kit obtained from Crystal
Chem, Inc, Downers Grove, Illinois. The primary antibodies of Thr-172
(phosphorylated AMPK) and protein phosphatase 2C-α (PP2C-α; nonphosphorylated
AMPK) were purchased from Abcam (Cambridge, Massachusetts). Total RNA extraction
kit and One-step Reverse transcription-polymerase chain reaction (RT-PCR) kit
was purchased from MP Biomedicals India Private Limited, India.

Pentylenetetrazole was purchased from Sigma Chemical Co. (St Louis, MO, USA). Total RNA Extraction kit and qRT-PCR (quantitative real time polymerase chain reaction) kit was purchased from MP Biomedicals India Private Limited, India. Microliter syringe (Hamilton, Bonaduz, Switzerland) was obtained from Anchrome Enterprises (I) Pvt. Ltd (Mumbai, India). Methanol, ethyl acetate, toluene, formic acid, precoated thin layer chromatographic (TLC) silica gel plates (Kieselgel 60, F-254, 0.2 mm) was purchased from Merck Life Science Pvt Ltd, India. High-performance thin-layer chromatography (HPTLC) spectra were recorded on a Linomat V (Camag, Muttenz, Switzerland).