Ligustilide

Ginsenoside rb1, neocryptotanshinone, caffeic acid and ligustilide (the purities of all standards were higher than 98% by high-performance liquid chromatography analysis) were purchased from Chengdu Pufeide Biotech Co., Ltd. (Chengdu, China).
TRIzol™ Reagent, Fetal bovine serum (FBS), 0.25% Trypsin–EDTA (w/v), Dulbecco’s modified eagle's medium (DMEM), penicillin–streptomycin (10,000 U/mL, P/S), and phosphate-buffered saline (PBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Human MTNR1A polyclonal antibody and GGT1 polyclonal antibody were purchased from CLOUD-CLONE CORP. (CCC, USA). Anti-rabbit IgG, HRP-linked antibody was purchased from Cell Signaling Technology (Danvers, MA, USA). β-actin was purchased from COHESION BIOSCIENCES (SUZHOU, CHINA).

RAS was collected from Min Country of Gansu Province, China, in November 2020. All of the plant materials were authenticated as A. sinensis (Oliv.) Diels by Professor Zhigang Yang. Polymerase chain reaction (PCR) tests were performed to identify the RAS (Liu et al., 2018 ). The PCR results are shown in Supplementary Figure S1. Voucher specimens (No. DG202009001) were stored at the School of Pharmacy, Lanzhou University. The weights per RAS for specimens falling into the first to fourth grades were more than 60.0 g, 25.0–60.0 g, 15.0–25.0 g, and 10.0–15.0 g, respectively.
Ferulic acid, ligustilide, senkyunolide I, chlorogenic acid, levistolide A, uridine, adenosine, guanine, and L-tryptophan were purchased from Chengdu Pufeide Biotech Co., Ltd. (Chengdu, China) as reference compounds. Acetonitrile and Methanol were purchased from CNW Technologies GmbH. Formic acid was purchased from Merck (Darmstadt, Germany). Double-distilled water (ddH₂O) was obtained using a laboratory water purifier. APH, CTX, and ATPase were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). adenosine diphosphate (ADP) and arachidonic acid (AA) were purchased from Shandong Tailixin Medical Technology Co., Ltd. (Shandong, China). Finally, aspirin was provided by the CSPC Pharmaceutical Group Co., Ltd.