N,N’ methylene bisacrylamide was from Promega (Madison, WI, USA). Molecular mass markers for SDS polyacrylamide gel electrophoresis (SDS PAGE) and nitrocellulose sheets (0.22 µm pores, 100 % nitrocellulose) were from GE Healthcare (Buckinghamshire, UK). Paraformaldehyde was from Electron Microscopy Sciences (Hatfield, PA, USA), and glutaraldehyde was from TAAB (Reading, UK). Sodium dodecyl sulfate (SDS) of high purity (>99 % C12 alkyl sulfate) was from Millipore (Carrigtwohill, C. Cork, Ireland). Electrophoresis equipment was from Hoefer Scientific Instruments (San Francisco, CA, USA). Permount mounting medium, ProLong anti-fade DAPI media, DyNAzyme II DNA Polymerase were from ThermoFisher Scientific (Waltham, Massachusetts, USA). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Electrophoresis equipment
Manufactured by Hoefer
Sourced in United States
Electrophoresis equipment is used to separate and analyze macromolecules, such as DNA, RNA, or proteins, based on their size and charge. It consists of a power supply, a gel chamber, and electrodes. The sample is loaded into the gel, and an electric current is applied, causing the molecules to migrate through the gel at different rates, separating them based on their physical properties.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose sheets, by GE Healthcare (2 mentions)
N n methylene bisacrylamide, by Promega (2 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (2 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (2 mentions)
Glutaraldehyde, by TAAB (2 mentions)
Dynazyme 2 dna polymerase, by Thermo Fisher Scientific (1 mentions)
Permount mounting medium, by Thermo Fisher Scientific (1 mentions)
3h gaba, by Cytiva (1 mentions)
2 protocols using electrophoresis equipment
1
SDS-PAGE and Immunoblotting Procedures
2
SDS-PAGE Reagents Protocol
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N,N' methylene bisacrylamide was from Promega (Madison, WI, USA). Molecular mass markers for SDS polyacrylamide gel electrophoresis (SDS PAGE) and nitrocellulose sheets (0.22 μm pores, 100 % nitrocellulose) were from GE Healthcare (Buckinghamshire, UK). Paraformaldehyde was from Electron Microscopy Sciences (Hatfield, PA, USA), and glutaraldehyde was from TAAB (Reading, UK). L-[ 3 H]glutamic acid (52 Ci/mmol) and [ 3 H]GABA (89 Ci/mmol) were from Amersham (Buckinghamshire, UK). Sodium dodecyl sulfate (SDS) of high purity (>99 % C12 alkyl sulfate) and nitrocellulose filters (HAWP; 0.45 μm pores) were from Millipore (Carrigtwohill, C. Cork, Ireland). Electrophoresis equipment was from Hoefer Scientific Instruments (San Francisco, CA, USA). All other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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