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Accuri cflow plus analysis software

Manufactured by BD

The BD Accuri CFlow Plus Analysis software is a flow cytometry data analysis tool. It provides users with the ability to analyze data generated from flow cytometry experiments.

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2 protocols using accuri cflow plus analysis software

1

Single Cell Flow Cytometry Protocol

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Single cell suspensions were prepared either from whole tumors as described above or from detached cell cultures as previously described by us 57 (link). Briefly, cells were washed with PBS and detached using accutase (Sigma) and then labeled with fluorophore-tagged antibodies. Cells were then washed and fixed in FACS buffer containing 1% paraformaldehyde. Cells were analyzed by flow cytometry using an Accuri C6 flow cytometer running BD Accuri CFlow Plus Analysis software. Samples were then analyzed using the FloJo software package (version 10).
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2

Single Cell Flow Cytometry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were prepared either from whole tumors as described above or from detached cell cultures as previously described by us 57 (link). Briefly, cells were washed with PBS and detached using accutase (Sigma) and then labeled with fluorophore-tagged antibodies. Cells were then washed and fixed in FACS buffer containing 1% paraformaldehyde. Cells were analyzed by flow cytometry using an Accuri C6 flow cytometer running BD Accuri CFlow Plus Analysis software. Samples were then analyzed using the FloJo software package (version 10).
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