Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in Surface Plasmon Resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
Biacore sensor chip nta
Manufactured by GE Healthcare
The Biacore-Sensor Chip NTA is a surface plasmon resonance (SPR) sensor chip designed for use with Biacore systems. The chip is coated with nitrilotriacetic acid (NTA), which can be used to immobilize metal-chelating proteins or peptides on the chip surface.
Automatically generated - may contain errors
Lab products found in correlation
Protein assay kit, by Bio-Rad (2 mentions)
Plasmid miniprep kit, by Qiagen (2 mentions)
Molecular biology grade reagents and chemicals, by Merck Group (2 mentions)
Thrombin cleavage capture kit, by Merck Group (2 mentions)
Amicon spin concentrator, by Merck Group (2 mentions)
Ni2 nta chromatography resin, by Merck Group (2 mentions)
Superdex 10 300 gl columns for akta fplc, by GE Healthcare (2 mentions)
Protein markers for sds page, by Bio-Rad (2 mentions)
2 protocols using biacore sensor chip nta
1
Protein Purification and Characterization
2
Protein Purification Using Ni2+ Chromatography
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Double distilled water was used in all experiments. Molecular biology grade chemicals and reagents were purchased from Sigma-Aldrich or Fisher Scientific. Ni2+-NTA chromatography resin and thrombin cleavage capture kits were purchased from EMD-Millipore. Superdex 10/300 GL columns for AKTA FPLC were purchased from GE Healthcare Life Sciences. Protein assay kits and protein markers for SDS-PAGE were purchased from Bio-Rad Laboratories. Amicon spin concentrators were purchased from EMD-Millipore. Plasmid MiniPrep Kits were purchased from Qiagen, and Biacore-Sensor Chip NTA and buffers used in surface plasmon resonance (SPR) analyses were purchased from GE Healthcare Life Sciences.
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