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Gm csf and il13

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GM-CSF is a cytokine that stimulates the production and differentiation of granulocytes and monocytes. IL13 is a cytokine that regulates immune and inflammatory responses.

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Lab products found in correlation

Draq7, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 conjugate, by Cell Signaling Technology (2 mentions) 24 well plate, by Corning (2 mentions) Lsrfortessa, by BD (2 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions) Foxp3 transcription factor permeabilization buffer, by Thermo Fisher Scientific (1 mentions)

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GM-CSF (148 citations)

2 protocols using gm csf and il13

1

Phospho-Akt and ERK1/2 Activation in mMDSCs and gMDSCs

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A total of 250,000 to 500,000 sorted mMDSCs and gMDSCs was cultured overnight in 10% RPMI supplemented with 50 ng/mL GM-CSF and IL13 (BioLegend) in 24-well plates (Corning Costar) and treated with 100 μg/mL sitagliptin. A fraction of the cells was stained with 1:1,000 diluted Draq 7 (Thermo Fisher Scientific) in PBS for 10 minutes at room temperature to assess viability using a BD LSR Fortessa. The remaining cells were incubated with 1.5% paraformaldehyde (Thermo Fisher Scientific) and 1× eBioscience Foxp3/transcription factor permeabilization buffer for 20 minutes at room temperature. Samples were stained with 1:50 diluted phospho-Akt (Ser473; D9E) XP Rabbit mAb (Alexa Fluor 647 Conjugate; Cell Signaling Technology, 4075S), phospho-p44/42 MAPK (ERK1/2; Thr202/Tyr204; E10) Mouse mAb (Alexa Fluor 647 Conjugate; Cell Signaling Technology, 4375S), or rabbit IgG Isotype Control (Alexa Fluor 647 Conjugate; Cell Signaling Technology, 3452S) in 1× eBioscience Foxp3/transcription factor permeabilization buffer for 1 hour at room temperature. Samples were acquired with a Cytek Aurora.
Bayik D., Bartels C.F., Lovrenert K., Watson D.C., Zhang D., Kay K., Lee J., Lauko A., Johnson S., Lo A., Silver D.J., McGraw M., Grabowski M., Mohammadi A.M., Veglia F., Fan Y., Vogelbaum M.A., Scacheri P, & Lathia J.D. (2022). Distinct Cell Adhesion Signature Defines Glioblastoma Myeloid-Derived Suppressor Cell Subsets. Cancer Research, 82(22), 4274-4287.
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2

Phosphorylation Analysis of mMDSCs and gMDSCs

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A total of 250,000–500,000 sorted mMDSCs and gMDSCs was cultured overnight in 10% RPMI supplemented with 50 ng/ml GM-CSF and IL-13 (Biolegend) in 24-well plates (Corning Costar) and treated with 100 μg/ml sitagliptin. A fraction of the cells was stained with 1:1000 diluted Draq 7 (Thermo Fisher Scientific) in PBS for 10 minutes at room temperature to assess viability using a BD LSR Fortessa. The remaining cells were incubated with 1.5% paraformaldehyde (Fisher Scientific) and 1x eBioscience Foxp3/Transcription Factor Permeabilization Buffer for 20 minutes at room temperature. Samples were stained with 1:50 diluted phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (Cell Signaling, 4075S), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (E10) Mouse mAb (Alexa Fluor® 647 Conjugate) (Cell Signaling, 4375S), or rabbit IgG Isotype Control (Alexa Fluor® 647 Conjugate) (Cell Signaling, 3452S) in 1x eBioscience Foxp3/Transcription Factor Permeabilization Buffer for 1 hour at room temperature. Samples were acquired with a Cytek Aurora.
Bayik D., Bartels C.F., Lovrenert K., Watson D.C., Zhang D., Kay K., Lee J., Lauko A., Johnson S., Lo A., Silver D.J., McGraw M., Grabowski M., Mohammadi A.M., Veglia F., Fan Y., Vogelbaum M.A., Scacheri P, & Lathia J.D. (2022). Distinct Cell Adhesion Signature Defines Glioblastoma Myeloid-Derived Suppressor Cell Subsets. Cancer Research, 82(22), 4274-4287.
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