Gs junior rapid library preparation kit

For whole genome shot gun sequencing, a Roche GS Junior platform (Roche, Basel, Schweiz) was employed using a GS Junior Rapid Library Preparation kit and GS Junior emPCR kit (Lib-L) according to the manufacture’s protocol. The read sequences were used to construct a contig without a reference sequence by de novo assembly using the GS De Novo Assembler (Roche, Basel, Schweiz). In this assembly, the program parameters were set to: seed step, 12; seed length, 16; seed count, 1; minimum overlap, 10; and minimum identity, 90.

Strains F2-382 and NIHS-28 were cultured overnight in brain-heart infusion broth (Eiken Chemical, Tochigi, Japan) at 37°C. Bacterial DNA was extracted using the phenol-chloroform extraction and ethanol precipitation methods [43 ,44 (link)]. For whole-genome shotgun sequencing, the GS Junior platform (Roche, Basel, Switzerland) was employed using a GS Junior Rapid Library Preparation Kit and a GS Junior emPCR Kit (Lib-L; Roche), according to the manufacturer’s protocol. The read sequences were used to construct a contig without a reference sequence by de novo assembly, using the GS De Novo Assembler (Roche). For this assembly, the program parameters were set to: seed step, 12; seed length, 16; seed count, 1; minimum overlap, 10; and minimum identity, 90.